TY - JOUR
T1 - Structure, chromosomal localization and expression of mouse genes encoding type III Reg, RegIIIα, RegIIIβ, RegIIIγ
AU - Narushima, Yoichi
AU - Unno, Michiaki
AU - Nakagawara, Kan Ichi
AU - Mori, Mitsuko
AU - Miyashita, Hikari
AU - Suzuki, Yoshihisa
AU - Noguchi, Naoya
AU - Takasawa, Shin
AU - Kumagai, Tomoko
AU - Yonekura, Hideto
AU - Okamoto, Hiroshi
N1 - Funding Information:
We are grateful to Mr. Masaki Mino and Mr. Katsuo Tsuchihara for their kind help, Mr. Hideo Kumagai for technical assistance and Mr. Brent Bell for critical reading of the manuscript. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan and Special Coordination Funds for Promoting Science and Technology from Science and Technology Agency, Japan, and is in partial fulfillment by Y.N. of the degree of Doctor of Medical Science at Tohoku University. M.U. and H.M. are recipients of fellowships from the Japan Society for Promotion of Science.
PY - 1997/2/7
Y1 - 1997/2/7
N2 - Reg (regenerating gene), first isolated from a rat regenerating islet cDNA library, is expressed in regenerating islet β-cells. Recently, it has been revealed that Reg and Reg-related genes constitute a multigene family, Reg family, which consists of three subtypes (type I, II, III) based on the primary structures of the encoded proteins of the genes. In mouse, type I and type II Reg genes (i.e. RegI and RegII gene) have so far been isolated. In the present study, the complete nucleotide (nt) sequences of the cDNAs and genes encoding murine type III Reg (regenerating gene product), RegIIIα, RegIIIβ and RegIIIγ were determined. RegIIIα, RegIIIβ and RegIIIγ encode 175-, 175- and 174-amino acid (aa) proteins, respectively, with 60-70% homology. All three genes are composed of six exons and five introns spanning approx. 3 kb, and exhibit distinctive structural features unique for members of the Reg gene family. All the mouse Reg genes, RegIIIα, RegIIIβ, RegIIIγ, RegI and RegII, are assigned to the adjacent site of chromosome 6C by fluorescence in situ hybridization (FISH). RegIIIα, RegIIIβ and RegIIIγ were expressed weakly in pancreas, strongly in intestinal tract, but not in hyperplastic islets, whereas both RegI and RegII were expressed in hyperplastic islets. These results suggest that genes of the mouse Reg family are derived from a common ancestor gene by several gene duplications, and have obtained divergency in expression and function in the process of genetic evolution.
AB - Reg (regenerating gene), first isolated from a rat regenerating islet cDNA library, is expressed in regenerating islet β-cells. Recently, it has been revealed that Reg and Reg-related genes constitute a multigene family, Reg family, which consists of three subtypes (type I, II, III) based on the primary structures of the encoded proteins of the genes. In mouse, type I and type II Reg genes (i.e. RegI and RegII gene) have so far been isolated. In the present study, the complete nucleotide (nt) sequences of the cDNAs and genes encoding murine type III Reg (regenerating gene product), RegIIIα, RegIIIβ and RegIIIγ were determined. RegIIIα, RegIIIβ and RegIIIγ encode 175-, 175- and 174-amino acid (aa) proteins, respectively, with 60-70% homology. All three genes are composed of six exons and five introns spanning approx. 3 kb, and exhibit distinctive structural features unique for members of the Reg gene family. All the mouse Reg genes, RegIIIα, RegIIIβ, RegIIIγ, RegI and RegII, are assigned to the adjacent site of chromosome 6C by fluorescence in situ hybridization (FISH). RegIIIα, RegIIIβ and RegIIIγ were expressed weakly in pancreas, strongly in intestinal tract, but not in hyperplastic islets, whereas both RegI and RegII were expressed in hyperplastic islets. These results suggest that genes of the mouse Reg family are derived from a common ancestor gene by several gene duplications, and have obtained divergency in expression and function in the process of genetic evolution.
KW - Cloning
KW - Fluorescence in situ hybridization
KW - Gene cluster
KW - Multigene family
KW - Nucleotide sequence
KW - Pancreas
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U2 - 10.1016/S0378-1119(96)00589-6
DO - 10.1016/S0378-1119(96)00589-6
M3 - Article
C2 - 9055810
AN - SCOPUS:0031045631
VL - 185
SP - 159
EP - 168
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -