Structure and alternative promoters of the mouse glutamic acid decarboxylase 67 gene

Yuchio Yanagawa, Takashi Kobayashi, Takashi Kamei, Kenji Ishii, Michiharu Nishijima, Akira Takaku, Takayasu Kobayashi, Shinri Tamura

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

γ-Aminobutyric acid is synthesized by glutamic acid decarboxylase (GAD), which has two forms, GAD65 and GAD67. Genomic clones coding mouse GAD67 (mGAD67) have been isolated. The restriction map of the overlapping clones covers a region of more than 45 kb of genomic DNA. The mGAD67 gene contains 16 translated exons in addition to an exon which is preferentially expressed in foetal brain. The rapid amplification of 5'-cDNA ends showed that mGAD67 gene transcripts have two different 5'-untranslated regions. Analysis of the genomic clones encompassing the 5'-exons revealed that the two transcripts arose from a single gene by alternative splicing using two different donor sites and a common acceptor. The exons were found 1.5 and 0.6 kb upstream of exon 1. The corresponding promoter regions of these exons have a number of putative regulatory elements, including Sp1- and Krox-24-binding sites. Analysis of mGAD67 transcripts demonstrated that each of the 5'-untranslated exons was expressed in mouse brain. In contrast, exon 0A, but not exon 0B, was expressed in mouse testis and pancreas. These results suggest that these transcripts may be regulated under the control of independent promoters.

Original languageEnglish
Pages (from-to)573-578
Number of pages6
JournalBiochemical Journal
Volume326
Issue number2
DOIs
Publication statusPublished - 1997 Sep 1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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