Structural reorganization of the bacterial cell-division protein FtsZ from Staphylococcus aureus

Takashi Matsui, Junji Yamane, Nobuyuki Mogi, Hiroto Yamaguchi, Hiroshi Takemoto, Min Yao, Isao Tanaka

    Research output: Contribution to journalArticle

    90 Citations (Scopus)

    Abstract

    FtsZ is a key molecule in bacterial cell division. In the presence of GTP, it polymerizes into tubulin-like proto-filaments by head-to-tail association. Protofilaments of FtsZ seem to adopt a straight or a curved conformation in relation to the bound nucleotide. However, although several bacterial and archaeal FtsZ structures have been determined, all of the structures reported previously are considered to have a curved conformation. In this study, structures of FtsZ from Staphylo-coccus aureus (SaFtsZ) were determined in apo, GDP-bound and inhibitor-complex forms and it was found that SaFtsZ undergoes marked conformational changes. The accumulated evidence suggests that the GDP-bound structure has the features of the straight form. The structural change between the curved and straight forms shows intriguing similarity to the eukaryotic cytoskeletal protein tubulin. Furthermore, the structure of the apo form showed an unexpectedly large conformational change in the core region. FtsZ has also been recognized as a novel target for antibacterial drugs. The structure of the complex with the inhibitor PC190723, which has potent and selective antistaphylococcal activity, indicated that the inhibitor binds at the cleft between the two subdomains.

    Original languageEnglish
    Pages (from-to)1175-1188
    Number of pages14
    JournalActa Crystallographica Section D: Biological Crystallography
    Volume68
    Issue number9
    DOIs
    Publication statusPublished - 2012 Sep

    Keywords

    • FtsZ
    • cell division
    • conformational change
    • inhibitor complex
    • tubulin

    ASJC Scopus subject areas

    • Structural Biology

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