Structural characterization of the O-antigenic polysaccharide chain of Porphyromonas circumdentaria NCTC 12469

Kouki Matsuo, Emiko Isogai, Yoshio Araki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Structural studies were carried out on an O-antigenic polysaccharide moiety derived from Porphyromonas circumdentaria NCTC 12469, a reference strain of Porphyromonas species. The polysaccharide chain was composed of D-glucose, D-galactose, N-acetyl-D-glucosamine, and N-acetyl-D-galactosamine in a molar ratio of 1:2:1:1. On the basis of results from 1H- and 13C-NMR spectroscopic analyses including COSY, TOCSY, and HMQC experiments together with results of Smith degradation, methylation analysis, and partial acid hydrolysis, it is concluded that the polysaccharide chain has a pentasaccharide repeating unit of →6)-β-D-Glcp-(1→6)-β-D-Galp-(1→6-β -D-Galp-(1→3)-β-D-GlcpNAc-(1→3)-β-D-GalpNAc-(1→. The immunoreaction between P. circumdentaria LPS and the corresponding antiserum was strongly inhibited by the pentasaccharide fragment (Glc-Gal-Gal-GlcNAc-GalNAc) isolated from partial acid hydrolysis of the above polysaccharide, suggestive of O-antigen specific antibodies in the used antiserum.

Original languageEnglish
Pages (from-to)299-306
Number of pages8
JournalMICROBIOLOGY and IMMUNOLOGY
Volume45
Issue number4
DOIs
Publication statusPublished - 2001

Keywords

  • Chemical structure
  • Lipopolysaccharide
  • O-antigenic polysaccharide
  • Porphyromonas circumdentaria

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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