Structural characterization of neutral and acidic oligosaccharides in the milks of strepsirrhine primates: Greater galago, aye-aye, Coquerel's sifaka and mongoose lemur

Epi Taufik, Kenji Fukuda, Akitsugu Senda, Tadao Saito, Cathy Williams, Chris Tilden, Regina Eisert, Olav Oftedal, Tadasu Urashima

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

The structures of milk oligosaccharides were characterized for four strepsirrhine primates to examine the extent to which they resemble milk oligosaccharides in other primates. Neutral and acidic oligosaccharides were isolated from milk of the greater galago (Galagidae: Otolemur crassicaudatus), aye-aye (Daubentoniidae: Daubentonia madagascariensis), Coquerel's sifaka (Indriidae: Propithecus coquereli) and mongoose lemur (Lemuridae: Eulemur mongoz), and their chemical structures were characterized by 1H-NMR spectroscopy. The oligosaccharide patterns observed among strepsirrhines did not appear to correlate to phylogeny, sociality or pattern of infant care. Both type I and type II neutral oligosaccharides were found in the milk of the aye-aye, but type II predominate over type I. Only type II oligosaccharides were identified in other strepsirrhine milks. α3-GL (isoglobotriose, Gal(α1-3)Gal(β1-4)Glc) was found in the milks of Coquerel's sifaka and mongoose lemur, which is the first report of this oligosaccharide in the milk of any primate species. 2-FL (Fuc(α1-2)Gal(β1-4)Glc) was found in the milk of an aye-aye with an ill infant. Oligosaccharides containing the Lewis x epitope were found in aye-aye and mongoose lemur milk. Among acidic oligosaccharides, 3-N-acetylneuraminyllactose (3-SL-NAc, Neu5Ac(α2-3)Gal (β1-4)Glc) was found in all studied species, whereas 6- Nacetylneuraminyllactose (6-SL-NAc, Neu5Ac(α2-6)Gal(β1- 4)Glc) was found in all species except greater galago. Greater galago milk also contained 3-N-glycolylneuraminyllactose (3-SL-NGc, Neu5Gc(α2-3)Gal(β1-4)Glc). The finding of a variety of neutral and acidic oligosaccharides in the milks of strepsirrhines, as previously reported for haplorhines, suggests that such constituents are ancient rather than derived features, and are as characteristic of primate lactation is the classic disaccharide, lactose.

Original languageEnglish
Pages (from-to)119-134
Number of pages16
JournalGlycoconjugate Journal
Volume29
Issue number2-3
DOIs
Publication statusPublished - 2012 Apr

Keywords

  • Aye-aye
  • Greater galago
  • Lactation
  • Lemur
  • Milk oligosaccharides
  • Phylogeny
  • Primates
  • Sifaka
  • Sociality
  • Strepsirrhines

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Structural characterization of neutral and acidic oligosaccharides in the milks of strepsirrhine primates: Greater galago, aye-aye, Coquerel's sifaka and mongoose lemur'. Together they form a unique fingerprint.

Cite this