Structural basis of [NiFe] hydrogenase maturation by Hyp proteins

Satoshi Watanabe, Daisuke Sasaki, Taiga Tominaga, Kunio Miki

Research output: Contribution to journalReview articlepeer-review

29 Citations (Scopus)


[NiFe] hydrogenases catalyze reversible hydrogen production/consumption. The active site of [NiFe] hydrogenases contains a complex NiFe(CN)2CO center, and the biosynthesis/maturation of these enzymes is a complex and dynamic process, primarily involving six Hyp proteins (HypABCDEF). HypA and HypB are involved in the Ni insertion, whereas the other four Hyp proteins (HypCDEF) are required for the biosynthesis, assembly and insertion of the Fe(CN) 2CO group. Over the last decades, a large number of functional and structural studies on maturation proteins have been performed, revealing detailed functions of each Hyp protein and the framework of the maturation pathway. This article will focus on recent advances in structural studies of the Hyp proteins and on mechanistic insights into the [NiFe] hydrogenase maturation.

Original languageEnglish
Pages (from-to)1089-1100
Number of pages12
JournalBiological Chemistry
Issue number10
Publication statusPublished - 2012 Oct
Externally publishedYes


  • Assembly of the metal center
  • Hydrogenase
  • Hyp protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry


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