Structural Basis of Backwards Motion in Kinesin-1-Kinesin-14 Chimera: Implication for Kinesin-14 Motility

Masahiko Yamagishi, Hideki Shigematsu, Takeshi Yokoyama, Masahide Kikkawa, Mitsuhiro Sugawa, Mari Aoki, Mikako Shirouzu, Junichiro Yajima, Ryo Nitta

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Kinesin-14 is a unique minus-end-directed microtubule-based motor. A swinging motion of a class-specific N-terminal neck helix has been proposed to produce minus-end directionality. However, it is unclear how swinging of the neck helix is driven by ATP hydrolysis utilizing the highly conserved catalytic core among all kinesins. Here, using a motility assay, we show that in addition to the neck helix, the conserved five residues at the C-terminal region in kinesin-14, namely the neck mimic, are necessary to give kinesin-1 an ability to reverse its directionality toward the minus end of microtubules. Our structural analyses further demonstrate that the C-terminal neck mimic, in cooperation with conformational changes in the catalytic core during ATP binding, forms a kinesin-14 bundle with the N-terminal neck helix to swing toward the minus end of microtubules. Thus, the neck mimic plays a crucial role in coupling the chemical ATPase reaction with the mechanical cycle to produce the minus-end-directed motility of kinesin-14.

Original languageEnglish
Pages (from-to)1322-1334
Number of pages13
JournalStructure
Volume24
Issue number8
DOIs
Publication statusPublished - 2016 Aug 2
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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