Structural basis for the high thermal stability and optimum pH of sphingomyelinase C from Streptomyces griseocarneus

Ikuhide Fujisawa, Hiroaki Hamana, Yu Tomita, Yusaku Matsumoto, Kazutaka Murayama, Daisuke Sugimori

Research output: Contribution to journalArticlepeer-review

Abstract

Sphingomyelinase C (SMC) hydrolyzes sphingomyelin to ceramide and phosphocholine. Prokaryotic SMCs share sequence homology with mammalian SMCs that have enzymatic pH optima at neutral pH. SMC from the nonpathogenic prokaryote Streptomyces griseocarneus shows notable enzymatic features such as higher optimum pH and thermostability than other prokaryotic SMCs. Determination of the three-dimensional structure of S. griseocarneus-SMC (Sg-SMC) and comparison with other SMC structures represents a promising strategy to elucidate the unique enzymatic features of Sg-SMC on a structural basis. Therefore, we determined the crystal structure of Sg-SMC at 2.0 Å resolution by X-ray crystallography. Comparison of the Sg-SMC structure with three other structurally known SMCs from Listeria ivanovii, Bacillus cereus, and Staphylococcus aureus indicated that Sg-SMC is more diverse in sequence and that structural differences in the main chain between these SMCs are primarily located on the molecular surface distant from the active site. Comparison of the surface area of the four SMCs revealed that Sg-SMC has the most compact structure, which may contribute to the enhanced thermostability of Sg-SMC. Regarding the hydrogen bond network in the active site of Sg-SMC, a basic amino acid, Arg278, is involved, whereas the corresponding residue in other SMCs (Ser or Asn) does not form hydrogen bonds with metal-coordinating water molecules. Hydrogen bond formation between Arg278 and a Mg2+ ion-coordinating water molecule may be responsible for the higher optimal pH of Sg-SMC compared to that of other SMCs.

Original languageEnglish
Pages (from-to)8-12
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume131
Issue number1
DOIs
Publication statusPublished - 2021 Jan

Keywords

  • Central metal-binding site
  • Coordinated water molecules
  • Hydrogen bond network
  • Sphingomyelinase C
  • Streptomyces griseocarneus
  • Structural diversity
  • Thermostability

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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