Structural basis for functional mimicry of long-variable-arm tRNA by transfer-messenger RNA

Yoshitaka Bessho, Rie Shibata, Shun Ichi Sekine, Kazutaka Murayama, Kyoko Higashijima, Chie Hori-Takemoto, Mikako Shirouzu, Seiki Kuramitsu, Shigeyuki Yokoyama

Research output: Contribution to journalArticlepeer-review

78 Citations (Scopus)

Abstract

tmRNA and small protein B (SmpB) are essential trans-translation system components. In the present study, we determined the crystal structure of SmpB in complex with the entire tRNA domain of the tmRNA from Thermus thermophilus. Overall, the ribonucleoprotein complex (tRNP) mimics a long-variable-arm tRNA (class II tRNA) in the canonical L-shaped tertiary structure. The tmRNA terminus corresponds to the acceptor and T arms, or the upper part, of tRNA. On the other hand, the SmpB protein simulates the lower part, the anticodon and D stems, of tRNA. Intriguingly, several amino acid residues collaborate with tmRNA bases to reproduce the canonical tRNA core layers. The linker helix of tmRNA had been considered to correspond to the anticodon stem, but the complex structure unambiguously shows that it corresponds to the tRNA variable arm. The tmRNA linker helix, as well as the long variable arm of class II tRNA, may occupy the gap between the large and small ribosomal subunits. This suggested how the tRNA domain is connected to the mRNA domain entering the mRNA channel. A loop of SmpB in the tRNP is likely to participate in the interaction with alanyl-tRNA synthetase, which may be the mechanism for the promotion of tmRNA alanylation by the SmpB protein. Therefore, the tRNP may simulate a tRNA, both structurally and functionally, with respect to aminoacylation and ribosome entry.

Original languageEnglish
Pages (from-to)8293-8298
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number20
DOIs
Publication statusPublished - 2007 May 15

Keywords

  • Crystal structure
  • Small protein B
  • Trans-translation
  • tmRNA

ASJC Scopus subject areas

  • General

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