Structural and mutational studies of anthocyanin malonyltransferases establish the features of BAHD enzyme catalysis

Hideaki Unno, Fumiko Ichimaida, Hirokazu Suzuki, Seiji Takahashi, Yoshikazu Tanaka, Atsushi Saito, Tokuzo Nishino, Masami Kusunoki, Toru Nakayama

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

The BAHD family is a class of acyl-CoA-dependent acyltransferases that are involved in plant secondary metabolism and show a diverse range of specificities for acyl acceptors. Anthocyanin acyltransferases make up an important class of the BAHD family and catalyze the acylation of anthocyanins that are responsible for most of the red-to-blue colors of flowers. Here, we describe crystallographic and mutational studies of three similar anthocyanin malonyltransferases from red chrysanthemum petals: anthocyanidin 3-O-glucoside-6″-O-malonyltransferase (Dm3MaT1), anthocyanidin 3-O-glucoside-3″, 6″-O-dimalonyltransferase (Dm3MaT2), and a homolog (Dm3MaT3). Mutational analyses revealed that seven amino acid residues in the N- and C-terminal regions are important for the differential acyl-acceptor specificity between Dm3MaT1 and Dm3MaT2. Crystallographic studies of Dm3MaT3 provided the first structure of a BAHD member, complexed with acyl-CoA, showing the detailed interactions between the enzyme and acyl-CoA molecules. The structure, combined with the results of mutational analyses, allowed us to identify the acyl-acceptor binding site of anthocyanin malonyltransferases, which is structurally different from the corresponding portion of vinorine synthase, another BAHD member, thus permitting the diversity of the acylacceptor specificity of BAHD family to be understood.

Original languageEnglish
Pages (from-to)15812-15822
Number of pages11
JournalJournal of Biological Chemistry
Volume282
Issue number21
DOIs
Publication statusPublished - 2007 May 25

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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