Structural and genomic DNA analysis of the putative TetR transcriptional repressor SCO7518 from Streptomyces coelicolor A3(2)

Takeshi Hayashi, Yoshikazu Tanaka, Naoki Sakai, Ui Okada, Min Yao, Nobuhisa Watanabe, Tomohiro Tamura, Isao Tanaka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

SCO7518 is a protein of unknown function from Streptomyces coelicolor A3(2) that has been classified into the TetR transcriptional regulator family. In this study, a crystal structure of SCO7518 was determined at 2.29 Å resolution. The structure is a homodimer of protomers that comprise an N-terminal DNA-binding domain and a C-terminal dimerization and regulatory domain, and possess a putative ligand-binding cavity. Genomic systematic evolution of ligands by exponential enrichment and electrophoretic mobility shift assays revealed that SCO7518 specifically binds to an operator sequence located upstream of the sco7519 gene, which encodes a maltose O-acetyltransferase. These results suggest that SCO7518 is a transcriptional repressor of sco7519 expression.

Original languageEnglish
Pages (from-to)4311-4318
Number of pages8
JournalFEBS Letters
Volume588
Issue number23
DOIs
Publication statusPublished - 2014 Nov 28

Keywords

  • Actinomycete
  • Genomic SELEX
  • Helix-turn-helix
  • TetR family
  • Transcriptional repressor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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