Structural and genetic characterization of fish skeletal muscle tropomyosins

Yoshihiro Ochiai, Shugo Watabe

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Tropomyosins were purified from the fast skeletal muscle of several fish species. While their behaviors on two-dimensional PAGE (20-PAGE) were examined, their thermodynamic properties were compared by differential scanning calorimetry (DSC). Furthermore, full- and partial lengths of cDNAs encoding fast skeletal muscle tropomyosins were cloned from white croaker and walleye pollack, respectively, and their nucleotide and deduced amino acid sequences were determined. It was suggested that the differences observed in the above two analytical parameters among fish species were due to only a few amino acid replacements in the molecule.

Original languageEnglish
Pages (from-to)1515-1518
Number of pages4
JournalFisheries Science
Volume68
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • DSC
  • cDNA cloning
  • fast skeletal muscle
  • fish
  • primary structure
  • tropomyosin

ASJC Scopus subject areas

  • Aquatic Science

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