TY - JOUR
T1 - Structural and functional characterization of gastric mucosa and central nervous system in histamine H2 receptor-null mice
AU - Fukushima, Yasushi
AU - Shindo, Takayuki
AU - Anai, Motonobu
AU - Saitoh, Toshihito
AU - Wang, Yuhui
AU - Fujishiro, Midori
AU - Ohashi, Yoshio
AU - Ogihara, Takehide
AU - Inukai, Kouichi
AU - Ono, Hiraku
AU - Sakoda, Hideyuki
AU - Kurihara, Yukiko
AU - Honda, Miho
AU - Shojima, Nobuhiro
AU - Fukushima, Harumi
AU - Haraikawa-Onishi, Yukiko
AU - Katagiri, Hideki
AU - Shimizu, Yasuhito
AU - Ichinose, Masao
AU - Ishikawa, Takashi
AU - Omata, Masao
AU - Nagai, Ryozo
AU - Kurihara, Hiroki
AU - Asano, Tomoichiro
PY - 2003/5/2
Y1 - 2003/5/2
N2 - To examine the physiological role of the histamine H2 receptor, histamine H2 receptor-null mice were generated by homologous recombination. Histamine H2 receptor-null mice, which developed normally and were fertile and healthy into adulthood, exhibited markedly enlarged stomachs and marked hypergastrinemia. The former was due to hyperplasia of gastric gland cells (small-sized parietal cells, enterochromaffin-like cells and mucous neck cells which were rich in mucin), but not of gastric surface mucous cells, which were not increased in number as compared with those in wild-type mice despite the marked hypergastrinemia. Basal gastric pH was slightly but significantly higher in histamine H2 receptor-null mice. Although carbachol but not gastrin induced in vivo gastric acid production in histamine H2 receptor-null mice, gastric pH was elevated by both muscarinic M3 and gastrin antagonists. Thus, both gastrin and muscarinic receptors appear to be directly involved in maintaining gastric pH in histamine H2 receptor-null mice. Interestingly, gastric glands from wild-type mice treated with an extremely high dose of subcutaneous lansoprazole (10 mg/kg body weight) for 3 months were very similar to those from histamine H2 receptor-null mice. Except for hyperplasia of gastric surface mucous cells, the findings for gastric glands from lansoprazole-treated wild-type mice were almost identical to those from gastric glands from histamine H2 receptor-null mice. Therefore, it is possible that the abnormal gastric glands in histamine H2 receptor-null mice are secondary to the severe impairment of gastric acid production, induced by the histamine H2 receptor disruption causing marked hypergastrinemia. Analyses of the central nervous system (CNS) of histamine H2 receptor-null mice revealed these mice to be different from wild-type mice in terms of spontaneous locomotor activity and higher thresholds for electrically induced convulsions. Taken together, these results suggest that (1) gastrin receptors are functional in parietal cells in histamine H2 receptor-null mice, (2) abnormal gastric glands in histamine H2 receptor-null mice may be secondary to severe impairment of gastric acid production and secretion and (3) histamine H2 receptors are functional in the central nervous system.
AB - To examine the physiological role of the histamine H2 receptor, histamine H2 receptor-null mice were generated by homologous recombination. Histamine H2 receptor-null mice, which developed normally and were fertile and healthy into adulthood, exhibited markedly enlarged stomachs and marked hypergastrinemia. The former was due to hyperplasia of gastric gland cells (small-sized parietal cells, enterochromaffin-like cells and mucous neck cells which were rich in mucin), but not of gastric surface mucous cells, which were not increased in number as compared with those in wild-type mice despite the marked hypergastrinemia. Basal gastric pH was slightly but significantly higher in histamine H2 receptor-null mice. Although carbachol but not gastrin induced in vivo gastric acid production in histamine H2 receptor-null mice, gastric pH was elevated by both muscarinic M3 and gastrin antagonists. Thus, both gastrin and muscarinic receptors appear to be directly involved in maintaining gastric pH in histamine H2 receptor-null mice. Interestingly, gastric glands from wild-type mice treated with an extremely high dose of subcutaneous lansoprazole (10 mg/kg body weight) for 3 months were very similar to those from histamine H2 receptor-null mice. Except for hyperplasia of gastric surface mucous cells, the findings for gastric glands from lansoprazole-treated wild-type mice were almost identical to those from gastric glands from histamine H2 receptor-null mice. Therefore, it is possible that the abnormal gastric glands in histamine H2 receptor-null mice are secondary to the severe impairment of gastric acid production, induced by the histamine H2 receptor disruption causing marked hypergastrinemia. Analyses of the central nervous system (CNS) of histamine H2 receptor-null mice revealed these mice to be different from wild-type mice in terms of spontaneous locomotor activity and higher thresholds for electrically induced convulsions. Taken together, these results suggest that (1) gastrin receptors are functional in parietal cells in histamine H2 receptor-null mice, (2) abnormal gastric glands in histamine H2 receptor-null mice may be secondary to severe impairment of gastric acid production and secretion and (3) histamine H2 receptors are functional in the central nervous system.
KW - Central nervous system
KW - Gastric mucosa
KW - Histamine H receptor
UR - http://www.scopus.com/inward/record.url?scp=0038404785&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038404785&partnerID=8YFLogxK
U2 - 10.1016/S0014-2999(03)01668-6
DO - 10.1016/S0014-2999(03)01668-6
M3 - Article
C2 - 12729842
AN - SCOPUS:0038404785
VL - 468
SP - 47
EP - 58
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1
ER -