Structural and functional analysis of a polyoma-related mammalian plasmid (L factor): The enhancer activity and plasmid establishment

H. Yoshimura, Y. Ikeda, M. Yoshimoto, S. Tamaki, K. Hanada, T. Kusano, T. Kohda, H. Saito, M. Oishi

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    Abstract

    L factor is a unique plasmid DNA which was originally discovered in a subclone (B822) of mouse L cells at a high copy number (more than 5,000 copies/cell). The presence of L factor caused no detectable abnormalities to the plasmid-bearing cells. We determined the total DNA sequence of the L factor I (and a part of L factor II) and compared it with that of polyoma DNA. Both DNA are common to the general construction of DNA frames such as early, late and non-coding regions, suggesting the two to be closely related. On the other hand, the L factor DNA sequences differ substantially from that of polyoma in the DNA sequences corresponding to the polyoma large T antigen, capsid proteins and a portion of the enhancer region. In order to investigate the mechanism of plasmid establishment of L factor, we compared the enhancer activity, capacity of DNA replication and efficiency of plasmid establishment of L factor with those of polyoma. The results indicate that L factor enhancer activity and DNA replication capacity were considerably lower than those of polyoma, suggesting that these altered (lowered) activities associated with L factor contribute to the plasmidal establishment and stable maintenance of L factor.

    Original languageEnglish
    Pages (from-to)3633-3639
    Number of pages7
    JournalNucleic acids research
    Volume19
    Issue number13
    DOIs
    Publication statusPublished - 1991 Jul 11

    ASJC Scopus subject areas

    • Genetics

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  • Cite this

    Yoshimura, H., Ikeda, Y., Yoshimoto, M., Tamaki, S., Hanada, K., Kusano, T., Kohda, T., Saito, H., & Oishi, M. (1991). Structural and functional analysis of a polyoma-related mammalian plasmid (L factor): The enhancer activity and plasmid establishment. Nucleic acids research, 19(13), 3633-3639. https://doi.org/10.1093/nar/19.13.3633