Structural and energetic hot-spots for the interaction between a ladder-like polycyclic ether and the anti-ciguatoxin antibody 10C9Fab

Mihoko Ui, Yoshikazu Tanaka, Takeshi Tsumuraya, Ikuo Fujii, Masayuki Inoue, Masahiro Hirama, Kouhei Tsumoto

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The mechanism by which anti-ciguatoxin antibody 10C9Fab recognizes a fragment of ciguatoxin CTX3C (CTX3C-ABCDE) was investigated by mutational analysis based on structural data. 10C9Fab has an extraordinarily large and deep antigen-binding pocket at the center of its variable region. We mutated several residues located at the antigen-binding pocket to Ala, and kinetic analysis of the interactions between the mutant proteins and the antigen fragment was performed. The results indicate that some residues associated with the rigid antigen-binding pocket are structural hot-spots and that L-N94 is an energetic hot-spot for association of the antibody with the antigen fragment CTX3C-ABCDE, suggesting the importance of structural complementarity and energetic hot-spot interactions for specific recognition of polycyclic ethers.

Original languageEnglish
Pages (from-to)793-798
Number of pages6
JournalMolecular BioSystems
Volume7
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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