Structural Analysis of a Lotus japonicus Genome. V. Sequence Features and Mapping of Sixty-four TAC Clones Which Cover the 6.4 Mb Regions of the Genome

Tomohiko Kato, Shusei Sato, Yasukazu Nakamura, Takakazu Kaneko, Erika Asamizu, Satoshi Tabata

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

We determined the nucleotide sequences of 64 TAC (transformation-competent artificial chromosome) clones selected from genomic libraries of Lotus japonicus accession Miyakojima MG-20 based on the sequence information of expressed sequence tags (ESTs), cDNAs, genes and DNA markers from L. japonicus and other legumes. The length of the DNA regions sequenced in this study was 6,370,255 bp, and the total length of the L. japonicus genome sequenced so far is 32,537,698 bp together with the nucleotide sequences of 256 TAC clones previously reported. Five hundred forty-eight potential-encoding genes with known or predicted functions, 127 gene segments and 224 pseudogenes were assigned to the newly sequenced regions by computer prediction and similarity searches against the sequences in protein and EST databases. Based on the nucleotide sequences of the clones, simple sequence repeat length polymorphism (SSLP) of derived cleaved amplified polymorphic sequence (dCAPS) markers were generated, and each clone was genetically localized onto the linkage map of two accessions of L. japonicus, MG-20 and Gifu B-129.

Original languageEnglish
Pages (from-to)277-285
Number of pages9
JournalDNA Research
Volume10
Issue number6
DOIs
Publication statusPublished - 2003 Dec 1
Externally publishedYes

Keywords

  • Gene prediction
  • Genomic sequence
  • Linkage mapping
  • Lotus japonicus
  • TAC genomic library

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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