Structural analysis of a Lotus japonicus genome. IV. Sequence features and mapping of seventy-three TAC clones which cover the 7.5 Mb regions of the genome

Erika Asamizu, Tomohiko Kato, Shusei Sato, Yasukazu Nakamura, Takakazu Kaneko, Satoshi Tabata

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Using the sequence information of expressed sequences tags (ESTs), cDNAs and genes from Lotus japonicus and other legumes, 73 TAC (transformation-competent artificial chromosomes) clones were selected from a genomic library of L. japonicus accession MG-20, and their nucleotide sequences were determined. The length of the DNA sequenced in this study was 7,455,959 bp, and the total length of the DNA regions sequenced so far is 26,167,443 bp together with the nucleotide sequences of 183 TAC clones previously reported. By similarity searches against the sequences in protein and EST databases and prediction by computer programs, a total of 699 potential protein-encoding genes with known or predicted functions, 163 gene segments and 267 pseudogenes were assigned to the newly sequenced regions. Based on the nucleotide sequences of the clones, simple sequence repeat length polymorphism (SSLP) or derived cleaved amplified polymorphic sequence (dCAPS) markers were generated, and each clone was located onto the linkage map of two accessions of L. japonicus, Gifu B-129 and Miyakojima MG-20. The sequence data, gene information and mapping information are available through the World Wide Web at http://www.kazusa.or.jp/lotus/.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalDNA Research
Volume10
Issue number3
DOIs
Publication statusPublished - 2003

Keywords

  • Gene prediction
  • Genomic sequence
  • Linkage mapping
  • Lotus japonicus
  • TAC genomic library

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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