Structural Alteration of Rice Pectin Affects Cell Wall Mechanical Strength and Pathogenicity of the Rice Blast Fungus under Weak Light Conditions

Takashi Ohara, Haruki Takeuchi, Junya Sato, Atsuko Nakamura, Hiroaki Ichikawa, Ryusuke Yokoyama, Kazuhiko Nishitani, Eiichi Minami, Shinobu Satoh, Hiroaki Iwai

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Pectin, a component of the plant cell wall, is involved in cell adhesion and environmental adaptations. We generated OsPG-FOX rice lines with little pectin due to overexpression of the gene encoding a pectin-degrading enzyme [polygalacturonase (PG)]. Overexpression of OsPG2 in rice under weak light conditions increased the activity of PG, which increased the degradation of pectin in the cell wall, thereby reducing adhesion. Under weak light conditions, the overexpression of OsPG decreased the pectin content and cell adhesion, resulting in abnormally large intercellular gaps and facilitating invasion by the rice blast fungus. OsPG2-FOX plants had weaker mechanical properties and greater sensitivity to biotic stresses than wild-type (WT) plants. However, the expression levels of disease resistance genes in non-infected leaves of OsPG2-FOX were more than twice as high as those of the WT and the intensity of disease symptoms was reduced, compared with the WT. Under normal light conditions, overexpression of OsPG2 decreased the pectin content, but did not affect cell adhesion and sensitivity to biotic stresses. Therefore, PG plays a role in regulating intercellular adhesion and the response to biotic stresses in rice.

Original languageEnglish
Pages (from-to)641-649
Number of pages9
JournalPlant and Cell Physiology
Volume62
Issue number4
DOIs
Publication statusPublished - 2021 Apr 1
Externally publishedYes

Keywords

  • Cell adhesion
  • Disease resistance
  • Oryza sativa
  • Pectin
  • Polygalacturonase

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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