Strong Telomerase Activity of B Lymphocyte from Mesenteric Lymph Nodes of Patients with Inflammatory Bowel Disease

Sho Takagi, Yoshitaka Kinouchi, Masaki Chida, Nobuo Hiwatashi, Mitsunori Noguchi, Seiichi Takahashi, Tooru Shimosegawa

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Telomerase acitivity can be induced in human B lymphocytes by in vitro stimulation of their antigen receptors. To determine whether telomerase activity is induced in vivo, we analyzed telomerase activity in B lymphocytes from the mesenteric lymph nodes of patients with inflammatory bowel disease (IBD), whose lymph nodes are well known to be strongly stimulated, and from those of non-inflamed controls. Seven IBD patients and 4 noninflamed controls were enrolled. Telomerase activity was assayed by telomeric repeat amplification protocol with minor modifications. The mesenteric lymph nodes from patients with IBD had stronger telomerase activity than those from controls or peripheral mononuclear cells. Isolation of CD19+ B lymphocytes from these lymph nodes showed that this strong activity resides in this lymphocytes subpopulation. This study provides the evidence that telomerase activity is induced in human B lymphocytes in human inflammatory disease.

Original languageEnglish
Pages (from-to)2091-2094
Number of pages4
JournalDigestive Diseases and Sciences
Volume48
Issue number10
DOIs
Publication statusPublished - 2003 Oct

Keywords

  • B lymphocytes
  • Inflammatory bowel disease
  • Telomerase
  • Telomeric repeat amplification protocol

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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