Strong Dependence of Hydration State of F-Actin on the Bound Mg2+/Ca2+ Ions

Makoto Suzuki, Asato Imao, George Mogami, Ryotaro Chishima, Takahiro Watanabe, Takaya Yamaguchi, Nobuyuki Morimoto, Tetsuichi Wazawa

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Understanding of the hydration state is an important issue in the chemomechanical energetics of versatile biological functions of polymerized actin (F-actin). In this study, hydration-state differences of F-actin by the bound divalent cations are revealed through precision microwave dielectric relaxation (DR) spectroscopy. G- and F-actin in Ca- and Mg-containing buffer solutions exhibit dual hydration components comprising restrained water with DR frequency f2 (<fw: DR frequency of bulk solvent, 17 GHz at 20 °C) and hypermobile water (HMW) with DR frequency f1 (>fw). The hydration state of F-actin is strongly dependent on the ionic composition. In every buffer tested, the HMW signal Dhyme (≡ (f1 - fw)1/(fww)) of F-actin is stronger than that of G-actin, where w is DR-amplitude of bulk solvent and 1 is that of HMW in a fixed-volume ellipsoid containing an F-actin and surrounding water in solution. Dhyme value of F-actin in Ca2.0-buffer (containing 2 mM Ca2+) is markedly higher than in Mg2.0-buffer (containing 2 mM Mg2+). Moreover, in the presence of 2 mM Mg2+, the hydration state of F-actin is changed by adding a small fraction of Ca2+ (∼0.1 mM) and becomes closer to that of the Ca-bound form in Ca2.0-buffer. This is consistent with the results of the partial specific volume and the Cotton effect around 290 nm in the CD spectra, indicating a change in the tertiary structure and less apparent change in the secondary structure of actin. The number of restrained water molecules per actin (N2) is estimated to be 1600-2100 for Ca2.0- and F-buffer and ∼2500 for Mg2.0-buffer at 10-15 °C. These numbers are comparable to those estimated from the available F-actin atomic structures as in the first water layer. The number of HMW molecules is roughly explained by the volume between the equipotential surface of -kT/2e and the first water layer of the actin surface by solving the Poisson-Boltzmann equation using UCSF Chimera.

Original languageEnglish
Pages (from-to)6917-6928
Number of pages12
JournalJournal of Physical Chemistry B
Volume120
Issue number28
DOIs
Publication statusPublished - 2016 Jul 21

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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