Strong Binding and Off–On Signaling Functions of Deep-Red Fluorescent TO-PRO-3 for Influenza A Virus RNA Promoter Region

Yusuke Sato, Yuri Aiba, Sayaka Yajima, Takaaki Tanabe, Kei Higuchi, Seiichi Nishizawa

Research output: Contribution to journalArticle

Abstract

The RNA promoter region of the influenza A virus has recently attracted much attention as an RNA target for the development of anti-influenza drugs. However, there are very few reports on small RNA-binding ligands targeting this region. In this work, it is reported that TO-PRO-3, a thiazole orange analogue with a trimethine bridge, exhibits strong and selective binding to the internal loop structure of the influenza A virus RNA promoter. This binding accompanies the remarkable light-up response of TO-PRO-3 in the deep-red spectral region. By virtue of these binding and fluorescence signaling functions, TO-PRO-3 can act as a useful indicator for the assessment of the binding capabilities of various test compounds for this RNA region, with a view toward the development of anti-influenza drug candidates.

Original languageEnglish
Pages (from-to)2752-2756
Number of pages5
JournalChemBioChem
Volume20
Issue number21
DOIs
Publication statusPublished - 2019 Nov 4

Keywords

  • RNA
  • drug discovery
  • dyes/pigments
  • fluorescence
  • viruses

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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