Abstract
The RNA promoter region of the influenza A virus has recently attracted much attention as an RNA target for the development of anti-influenza drugs. However, there are very few reports on small RNA-binding ligands targeting this region. In this work, it is reported that TO-PRO-3, a thiazole orange analogue with a trimethine bridge, exhibits strong and selective binding to the internal loop structure of the influenza A virus RNA promoter. This binding accompanies the remarkable light-up response of TO-PRO-3 in the deep-red spectral region. By virtue of these binding and fluorescence signaling functions, TO-PRO-3 can act as a useful indicator for the assessment of the binding capabilities of various test compounds for this RNA region, with a view toward the development of anti-influenza drug candidates.
Original language | English |
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Pages (from-to) | 2752-2756 |
Number of pages | 5 |
Journal | ChemBioChem |
Volume | 20 |
Issue number | 21 |
DOIs | |
Publication status | Published - 2019 Nov 4 |
Keywords
- RNA
- drug discovery
- dyes/pigments
- fluorescence
- viruses
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Organic Chemistry