Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: Simultaneous binding to DNA phosphate backbone

Chunxia Zhao; Qing Dai; Takehiro Seino; Ying Yu Cui; Seiichi Nishizawa; Norio Teramae

doi:10.1039/b516575j

Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: Simultaneous binding to DNA phosphate backbone

Chunxia Zhao, Qing Dai, Takehiro Seino, Ying Yu Cui, Seiichi Nishizawa, Norio Teramae

SCI - Chemistry

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.

Original language	English
Pages (from-to)	1185-1187
Number of pages	3
Journal	Chemical Communications
Issue number	11
DOIs	https://doi.org/10.1039/b516575j
Publication status	Published - 2006 Mar 13

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/b516575j

Cite this

@article{39238385c685400388ba52558292e79c,

title = "Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: Simultaneous binding to DNA phosphate backbone",

abstract = "Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.",

author = "Chunxia Zhao and Qing Dai and Takehiro Seino and Cui, {Ying Yu} and Seiichi Nishizawa and Norio Teramae",

year = "2006",

month = mar,

day = "13",

doi = "10.1039/b516575j",

language = "English",

pages = "1185--1187",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "11",

}

TY - JOUR

T1 - Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes

T2 - Simultaneous binding to DNA phosphate backbone

AU - Zhao, Chunxia

AU - Dai, Qing

AU - Seino, Takehiro

AU - Cui, Ying Yu

AU - Nishizawa, Seiichi

AU - Teramae, Norio

PY - 2006/3/13

Y1 - 2006/3/13

N2 - Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.

AB - Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.

UR - http://www.scopus.com/inward/record.url?scp=33644777816&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644777816&partnerID=8YFLogxK

U2 - 10.1039/b516575j

DO - 10.1039/b516575j

M3 - Article

C2 - 16518485

AN - SCOPUS:33644777816

SP - 1185

EP - 1187

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 11

ER -

Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: Simultaneous binding to DNA phosphate backbone

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this