Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: Simultaneous binding to DNA phosphate backbone

Chunxia Zhao; Qing Dai; Takehiro Seino; Ying Yu Cui; Seiichi Nishizawa; Norio Teramae

doi:10.1039/b516575j

Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: Simultaneous binding to DNA phosphate backbone

Chunxia Zhao, Qing Dai, Takehiro Seino, Ying Yu Cui, Seiichi Nishizawa, Norio Teramae

SCI - Chemistry

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.

Original language	English
Pages (from-to)	1185-1187
Number of pages	3
Journal	Chemical Communications
Issue number	11
DOIs	https://doi.org/10.1039/b516575j
Publication status	Published - 2006 Mar 13

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.",

author = "Chunxia Zhao and Qing Dai and Takehiro Seino and Cui, {Ying Yu} and Seiichi Nishizawa and Norio Teramae",

year = "2006",

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doi = "10.1039/b516575j",

language = "English",

pages = "1185--1187",

journal = "Chemical Communications",

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T1 - Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes

T2 - Simultaneous binding to DNA phosphate backbone

AU - Zhao, Chunxia

AU - Dai, Qing

AU - Seino, Takehiro

AU - Cui, Ying Yu

AU - Nishizawa, Seiichi

AU - Teramae, Norio

PY - 2006/3/13

Y1 - 2006/3/13

N2 - Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.

AB - Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.

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JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 11

ER -

Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: Simultaneous binding to DNA phosphate backbone

Abstract

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