Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: Simultaneous binding to DNA phosphate backbone

Chunxia Zhao, Qing Dai, Takehiro Seino, Ying Yu Cui, Seiichi Nishizawa, Norio Teramae

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.

Original languageEnglish
Pages (from-to)1185-1187
Number of pages3
JournalChemical Communications
Issue number11
DOIs
Publication statusPublished - 2006 Mar 13

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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