Strong and selective binding of amiloride to an abasic site in RNA duplexes: Thermodynamic characterization and microRNA detection

Yusuke Sato; Toshiki Ichihashi; Seiichi Nishizawa; Norio Teramae

doi:10.1002/anie.201201790

Strong and selective binding of amiloride to an abasic site in RNA duplexes: Thermodynamic characterization and microRNA detection

Yusuke Sato, Toshiki Ichihashi, Seiichi Nishizawa, Norio Teramae

SCI - Chemistry

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Firmly tied: The binding affinity of amiloride for an abasic (AP) site-containing RNA duplex (see picture) is two orders of magnitude superior to the affinity of the corresponding AP site-containing DNA duplex. The observed high binding affinity for the RNA duplex arises from a favorable enthalpy gain. The binding-induced fluorescence response of amiloride is applicable to microRNA detection.

Original language	English
Pages (from-to)	6369-6372
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	26
DOIs	https://doi.org/10.1002/anie.201201790
Publication status	Published - 2012 Jun 25

Keywords

RNA
abasic sites
fluorescence
thermodynamics

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201201790

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AU - Nishizawa, Seiichi

AU - Teramae, Norio

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KW - fluorescence

KW - thermodynamics

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