Firmly tied: The binding affinity of amiloride for an abasic (AP) site-containing RNA duplex (see picture) is two orders of magnitude superior to the affinity of the corresponding AP site-containing DNA duplex. The observed high binding affinity for the RNA duplex arises from a favorable enthalpy gain. The binding-induced fluorescence response of amiloride is applicable to microRNA detection.
- abasic sites
ASJC Scopus subject areas