Strong and selective binding of amiloride to an abasic site in RNA duplexes: Thermodynamic characterization and microRNA detection

Yusuke Sato, Toshiki Ichihashi, Seiichi Nishizawa, Norio Teramae

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

Firmly tied: The binding affinity of amiloride for an abasic (AP) site-containing RNA duplex (see picture) is two orders of magnitude superior to the affinity of the corresponding AP site-containing DNA duplex. The observed high binding affinity for the RNA duplex arises from a favorable enthalpy gain. The binding-induced fluorescence response of amiloride is applicable to microRNA detection.

Original languageEnglish
Pages (from-to)6369-6372
Number of pages4
JournalAngewandte Chemie - International Edition
Volume51
Issue number26
DOIs
Publication statusPublished - 2012 Jun 25

Keywords

  • RNA
  • abasic sites
  • fluorescence
  • thermodynamics

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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