Abstract
Firmly tied: The binding affinity of amiloride for an abasic (AP) site-containing RNA duplex (see picture) is two orders of magnitude superior to the affinity of the corresponding AP site-containing DNA duplex. The observed high binding affinity for the RNA duplex arises from a favorable enthalpy gain. The binding-induced fluorescence response of amiloride is applicable to microRNA detection.
Original language | English |
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Pages (from-to) | 6369-6372 |
Number of pages | 4 |
Journal | Angewandte Chemie - International Edition |
Volume | 51 |
Issue number | 26 |
DOIs | |
Publication status | Published - 2012 Jun 25 |
Keywords
- RNA
- abasic sites
- fluorescence
- thermodynamics
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)