TY - JOUR
T1 - Stretching of single DNA molecules complexed with restriction endonuclease by Langmuir-Blodgett method
AU - Matsuo, Yasutaka
AU - Ijiro, Kuniharu
AU - Shimomura, Masatsugu
N1 - Funding Information:
This work was financially supported by the Precursory Research for Embryonic Science and Technology project (PRESTO) (Conversion and Control by Advanced Chemistry) of Japan Science and Technology Corporation (JST) and also by a Grant-in-Aid on Scientific Research from the Ministry of Education, Science, Sport, and Culture of Japan.
PY - 2005/2/25
Y1 - 2005/2/25
N2 - We have proposed a new technique for stretching single double-stranded DNA molecules on solid substrates by the Langmuir-Blodgett (LB) method. The polyion complex monolayer of a cationic amphiphile and DNA molecules formed at the air-water interface was transferred on a clean glass substrate. Vertical lifting up of the glass substrate provided the transferred monolayer consisting the stretched individual DNA molecules aligned parallel to the lifting direction on the glass. The DNA molecules complexed with the restriction endonuclease (EcoRI) were employed for stretching by using this method. Fluorescence images of the transferred monolayer showed that the EcoRI-binding DNA molecules could be stretched and immobilized on the glass substrate. A specific sequence of DNA recognized by EcoRI was detected as spatial positions of the stretched DNA molecules.
AB - We have proposed a new technique for stretching single double-stranded DNA molecules on solid substrates by the Langmuir-Blodgett (LB) method. The polyion complex monolayer of a cationic amphiphile and DNA molecules formed at the air-water interface was transferred on a clean glass substrate. Vertical lifting up of the glass substrate provided the transferred monolayer consisting the stretched individual DNA molecules aligned parallel to the lifting direction on the glass. The DNA molecules complexed with the restriction endonuclease (EcoRI) were employed for stretching by using this method. Fluorescence images of the transferred monolayer showed that the EcoRI-binding DNA molecules could be stretched and immobilized on the glass substrate. A specific sequence of DNA recognized by EcoRI was detected as spatial positions of the stretched DNA molecules.
KW - DNA
KW - Langmuir-Blodgett method
KW - Polyion complex monolayer
KW - Restriction enzyme
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U2 - 10.1016/j.colsurfb.2004.10.025
DO - 10.1016/j.colsurfb.2004.10.025
M3 - Article
C2 - 15708499
AN - SCOPUS:13644253048
VL - 40
SP - 123
EP - 126
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
IS - 3-4 SPEC. ISS.
ER -