Stress relaxation measurement of fibroblast cells with atomic force microscopy

Takaharu Okajima, Masara Tanaka, Shusaku Tsukiyama, Tsubasa Kadowaki, Sadaaki Yamamoto, Masatsugu Shimomura, Hiroshi Tokumoto

    Research output: Contribution to journalArticlepeer-review

    19 Citations (Scopus)


    We measured the stress relaxation of mouse fibroblast NIH3T3 cells with an atomic force microscope (AFM) using a sharp silicon tip and a silica bead with a radius of ∼1 μm as an indenter. The decay of loading force was clearly observed in NIH3T3 cells at a small initial loading force of ∼0.4 nN and was well fitted to the stretched exponential function rather than to a single exponential function. The stretching exponent parameter was ∼0.5 for both indenters, indicating that the stress relaxation observed in NIH3T3 cells consisted of multiple relaxation processes. The time-domain AFM technique described in this report allows us to measure directly the relaxation process of living cells in a range from milliseconds to seconds.

    Original languageEnglish
    Pages (from-to)5552-5555
    Number of pages4
    JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
    Issue number8 B
    Publication statusPublished - 2007 Aug 23


    • Atomic force microscope
    • Colloidal probe
    • Living cells
    • Stress relaxation
    • Viscoelastic properties

    ASJC Scopus subject areas

    • Engineering(all)
    • Physics and Astronomy(all)

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