TY - JOUR
T1 - Strategies for the stable performance and rapid inhibition recovery of a thermophilic digester treating coffee wastes and the synergistic effects of microbes
AU - Li, Qian
AU - Cheng, Xinru
AU - Yuwen, Chaosui
AU - Yang, Xiaohuan
AU - Qiao, Wei
AU - Li, Yu You
AU - Wang, Xiaochang
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant No. 51608430 ), Scientific Research Program Funded by the Shaanxi Provincial Education Department (Grant No. 17JS077 ), and Natural Science Foundation for Young Scientists of Xi'an University of Architecture and Technology, China (Grant No. QN1615 ).
Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant No. 51608430), Scientific Research Program Funded by the Shaanxi Provincial Education Department (Grant No. 17JS077), and Natural Science Foundation for Young Scientists of Xi'an University of Architecture and Technology, China (Grant No. QN1615).
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/8
Y1 - 2018/8
N2 - A 200-day experiment was conducted to evaluate strategies for the stable performance and rapid inhibition recovery of a thermophilic reactor treating coffee wastes, waste activated sludge, and milk waste under a high organic loading rate (OLR) and the synergistic effects of microbes. Excessive accumulation of propionate was determined to be the main reason for the deterioration of the reactor, which resulted from reduced methanogenic activity. Supplementation of inoculum sludge led to the complete inhibition recovery of the reactor in fewer than 35 d by enhancing propionate degradation. The microelement and low concentration of sulfate ensured the stable performance of the reactor at OLR of 4.6 g-TS/L/d. The efficiency of organic matters and propionate degradation was also improved. The microbial community was analyzed by high-throughput sequencing targeting 16S rRNA to determine the relationship between microbial composition and reactor performance. Propionate markedly inhibited Methanosarcina, which dominated the stable state; meanwhile, Methanobacterium and Methanothermobacter were more tolerant and comprised more than 75% of total archaea at the inhibitory stage. The deterioration of the reactor led to a shift in the CH4 generation pathway from acetoclastic to hydrogenotrophic. Clostridiales was responsible for organic degradation at stable stages; however, almost all of the genera of Clostridiales could not survive at stage II and were replaced by Thermoanaerobacterales and Lactobacillales.
AB - A 200-day experiment was conducted to evaluate strategies for the stable performance and rapid inhibition recovery of a thermophilic reactor treating coffee wastes, waste activated sludge, and milk waste under a high organic loading rate (OLR) and the synergistic effects of microbes. Excessive accumulation of propionate was determined to be the main reason for the deterioration of the reactor, which resulted from reduced methanogenic activity. Supplementation of inoculum sludge led to the complete inhibition recovery of the reactor in fewer than 35 d by enhancing propionate degradation. The microelement and low concentration of sulfate ensured the stable performance of the reactor at OLR of 4.6 g-TS/L/d. The efficiency of organic matters and propionate degradation was also improved. The microbial community was analyzed by high-throughput sequencing targeting 16S rRNA to determine the relationship between microbial composition and reactor performance. Propionate markedly inhibited Methanosarcina, which dominated the stable state; meanwhile, Methanobacterium and Methanothermobacter were more tolerant and comprised more than 75% of total archaea at the inhibitory stage. The deterioration of the reactor led to a shift in the CH4 generation pathway from acetoclastic to hydrogenotrophic. Clostridiales was responsible for organic degradation at stable stages; however, almost all of the genera of Clostridiales could not survive at stage II and were replaced by Thermoanaerobacterales and Lactobacillales.
KW - Coffee grounds
KW - Microbial community
KW - Propionate degradation
KW - Thermophilic co-digestion
KW - Waste activated sludge
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U2 - 10.1016/j.ibiod.2018.02.014
DO - 10.1016/j.ibiod.2018.02.014
M3 - Article
AN - SCOPUS:85044297586
VL - 132
SP - 114
EP - 121
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
SN - 0964-8305
ER -