Storage of GABA in chromaffin granules and not in synaptic-like microvesicles in rat adrenal medullary cells

Keita Harada, Hidetada Matsuoka, Jun Nakamura, Mitsunori Fukuda, Masumi Inoue

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    Neurons and certain kinds of endocrine cells, such as adrenal medullary (AM) cells, have large dense-core vesicles (LDCVs) and synaptic vesicles or synaptic-like microvesicles (SLMVs). These secretory vesicles differ in Ca 2+ sensitivity and contain different signaling substances. We have recently reported that GABA functions as a paracrine factor in rat AM cells and modulates catecholamine secretion. The present experiment was undertaken to examine the subcellular localization of the GABA system including GABA itself in AM cells. Fractionation analysis with sucrose density gradient and immunocytochemistry indicated that vesicular GABA transporter (VGAT) was localized in LDCVs and not SLMVs in rat and bovine AM cells. In addition, significant amounts of GABA were detected in high density fractions, which contained LDCVs. When green fluorescence protein-VGAT and green fluorescence protein-vesicular ACh transporter were exogenously expressed in PC12 cells, the former and the latter were selectively targeted to LDCVs and SLMVs, respectively. We conclude that GABA is stored in chromaffin granules in rat and bovine AM cells through VGAT.

    Original languageEnglish
    Pages (from-to)617-626
    Number of pages10
    JournalJournal of Neurochemistry
    Volume114
    Issue number2
    DOIs
    Publication statusPublished - 2010 Jul 1

    Keywords

    • GABA transporter
    • PC12
    • chromaffin cell
    • chromaffin granules
    • synaptic-like microvesicles
    • γ-aminobutyric acid

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

    Fingerprint Dive into the research topics of 'Storage of GABA in chromaffin granules and not in synaptic-like microvesicles in rat adrenal medullary cells'. Together they form a unique fingerprint.

    Cite this