Stoichiometric growth model for riboflavin-producing Bacillus subtilis

Toshiaki Mori, Shintaro Kishimoto, Kuniharu Ijiro, Atsushi Kobayashi, Yoshio Okahata

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Rate equations for measured extracellular rates and macromolecular composition data were combined with a stoichiometric model to describe riboflavin production with an industrial Bacillus subtilis strain using errors in variables regression analysis. On the basis of this combined stoichiometric growth model, we explored the topological features of the B. subtilis metabolic reaction network that was assembled from a large amount of literature. More specifically, we simulated maximum theoretical yields of biomass and riboflavin, including the associated flux regimes. Based on the developed model, the importance of experimental data on building block requirements for maximum yield and flux calculations were investigated. These analyses clearly show that verification of macromolecular composition data is important for optimum flux calculations.

Original languageEnglish
Pages (from-to)132-143
Number of pages12
JournalBiotechnology and Bioengineering
Volume76
Issue number2
DOIs
Publication statusPublished - 2001 Jan 1

Keywords

  • Bacillus subtilis
  • Metabolic flux
  • Riboflavin production
  • Stoichiometry

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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    Mori, T., Kishimoto, S., Ijiro, K., Kobayashi, A., & Okahata, Y. (2001). Stoichiometric growth model for riboflavin-producing Bacillus subtilis. Biotechnology and Bioengineering, 76(2), 132-143. https://doi.org/10.1002/bit.1153