Stimulation of the phage λ pL promoter by integration host factor requires the carboxy terminus of the α-subunit of RNA polymerase

Hilla Giladi, Kazuhiko Igarashi, Akira Ishihama, Amos B. Oppenheim

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Escherichia coli integration host factor (IHF) binds with high affinity to two tandem IHF consensus sequences located upstream from the pL promoter of bacteriophage lambda. IHF was shown to stimulate transcription initiation from the pL promoter by increasing close complex formation (KB). We show here, by the use of reconstituted mutant RNA polymerases, that the C-terminal portion of the α subunit of RNA polymerase plays an essential role in the stimulation of transcription by IHF. Our results are in agreement with the hypothesis that IHF, like the cAMP-CRP activator, increases the affinity of RNA polymerase to the promoter by protein-protein interaction.

Original languageEnglish
Pages (from-to)985-990
Number of pages6
JournalJournal of Molecular Biology
Volume227
Issue number4
DOIs
Publication statusPublished - 1992 Oct 20
Externally publishedYes

Keywords

  • Escherichia coli
  • IHF
  • RNA polymerase
  • phage λ
  • transcription initiation

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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