Stimulation of splenocytes in C3H/HeJ mice with Porphyromonas gingivalis lipid A in comparison with enterobacterial lipid A

Tomohiko Ogawa, Hidetoshi Shimauchi, Hiroshi Uchida, Yoshihide Mori

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Porphyromonas gingivalis 381 lipid A induced strong mitogenic response in splenic B cells separated from LPS-non-responsive C3H/HeJ mice as well as LPS-responsive C3H/HeN mice, by using a magnetic cell sorting system. The lipid A also exhibited mitogenic activity in splenic T cells. P. gingivalis lipid A induced lower production of interleukin-1β (IL-1β) in splenic macrophage cultures and exhibited a comparable IL-6 producing activity in splenic B cells of C3H/HeN mice as compared to Escherichia coli-type synthetic lipid A (compound 506) and monophosphoryl lipid A from Salmonella minnesota Re 595 (MLA). Furthermore, P. gingivalis lipid A, but not compound 506 nor MLA, induced low IL-1β and high IL-6 production in C3H/HeJ mice. C3H/HeJ T cells in response to P. gingivalis lipid A stimulation resulted in definite IL-2 mRNA and its production, whereas IL-4 response was scarcely active in both C3H/HeN and C3H/HeJ T cells. P. gingivalis lipid A increased tyrosine, serine/threonine phosphorylation of proteins with various major induced bands in splenic B cells of both C3H/HeN and C3H/HeJ mice. Thus, P. gingivalis lipid A stimulated the splenic immunocytes of C3H/HeJ as well as C3H/HeN mice.

Original languageEnglish
Pages (from-to)399-414
Number of pages16
JournalImmunobiology
Volume196
Issue number4
DOIs
Publication statusPublished - 1996 Jan 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

Fingerprint Dive into the research topics of 'Stimulation of splenocytes in C3H/HeJ mice with Porphyromonas gingivalis lipid A in comparison with enterobacterial lipid A'. Together they form a unique fingerprint.

  • Cite this