Stimulation of muscarinic receptor in hippocampal neuron induces characteristic increase in cytosolic free Ca2+ concentration

Yoshihisa Kudo, Akihiko Ogura, Toshio Iijima

    Research output: Contribution to journalArticlepeer-review

    35 Citations (Scopus)

    Abstract

    Changes in cytosolic free Ca2+ concentration ([Ca2+]i) in response to acetylcholine (ACh) were examined by fura-2 fluorometry in cultured rat hippocampal neurons. ACh (≧10-5 M) induced an increase in [Ca2+]i composed of fast transient and slow long-lasting phases. Atropine (10-8 M) abolished the fast component and greatly reduced the slow component. The slow component was selectively blocked by pirenzepine (10-6 M). The effect of ACh remained partially in a Ca2+-deficient medium where effects of l-glutamate and KCl (50 mM) were abolished. Present results suggest that ACh elevates [Ca2+]i by activation of muscarinic receptor subtypes, one of which is coupled with ion channels and the other of which transduces the ACh binding to mobilization of intracellularly stored Ca2+.

    Original languageEnglish
    Pages (from-to)345-350
    Number of pages6
    JournalNeuroscience Letters
    Volume85
    Issue number3
    DOIs
    Publication statusPublished - 1988 Mar 10

    Keywords

    • Acetylcholine
    • Fura-2 fluorometry
    • Hippocampal neuron
    • Muscarinic receptor

    ASJC Scopus subject areas

    • Neuroscience(all)

    Fingerprint

    Dive into the research topics of 'Stimulation of muscarinic receptor in hippocampal neuron induces characteristic increase in cytosolic free Ca<sup>2+</sup> concentration'. Together they form a unique fingerprint.

    Cite this