Stimulation of emergence of root apical meristems in horseradish hairy root by auxin supplementation and its kinetic model

Yutaka Nakashimada, Nobuyuki Uozumi, Takeshi Kobayashi

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

1-Naphthaleneacetic acid (NAA) in the medium strongly inhibited horseradish hairy root elongation. In hatch culture, NAA in the medium was absorbed by the root at the beginning of the culture (0-4 d). After the depletion of NAA, root apical meristems emerged, and then the growth rate of the root increased dramatically. The batch culture with 1 × 10-3 kg/m3 of NAA reached the highest biomass, 10.9 kg-dry weight/m3 at 21 d. Modification of a kinetic model previously developed resulted in a favorable fit with the growth curve of horseradish hairy root treated with NAA. Furthermore, in repeated batch culture, the NAA-treated root culture exhibited a 1.7-fold increase in dry weight, 57 kg/m3 at 40 d, compared with that of no NAA-treated root. The modified kinetic model also correlated well with the experimental results in repeated batch culture.

Original languageEnglish
Pages (from-to)178-182
Number of pages5
JournalJournal of Fermentation and Bioengineering
Volume77
Issue number2
DOIs
Publication statusPublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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