Stimulation of DNA-dependent protein kinase activity by high mobility group proteins 1 and 2

Fumiaki Watanabe, Hitoshi Shirakawa, Michiteru Yoshida, Kinji Tsukada, Hirobumi Teraoka

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

After incubation of high mobility group (HMG) proteins 1 and 2 with DNA-dependent protein kinase (DNA-PK) and [γ-32P]ATP in the presence of double-stranded DNA, not only phosphorylation of HMG proteins but also enhancement of autophosphorylation of the catalytic of 350 kDa in DNA-PK was observed. DNA-PK activity with a synthetic peptide and α-casein as substrates was stimulated several-fold by HMG1, HMG2, and the DNA-binding domains. The stimulation was decreased at higher concentrations of HMG proteins, and DNA-PK activity was inhibited by histone H1. Electrophoretic mobility shift analysis suggests that HMG proteins facilitate the binding of DNA-PK to DNA.

Original languageEnglish
Pages (from-to)736-742
Number of pages7
JournalBiochemical and biophysical research communications
Volume202
Issue number2
DOIs
Publication statusPublished - 1994 Jul 29
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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