Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein

Naoyuki Sarai, Wataru Kagawa, Takashi Kinebuchi, Ako Kagawa, Kozo Tanaka, Kiyoshi Miyagawa, Shukuko Ikawa, Takehiko Shibata, Hitoshi Kurumizaka, Shigeyuki Yokoyama

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.

Original languageEnglish
Pages (from-to)4429-4437
Number of pages9
JournalNucleic acids research
Volume34
Issue number16
DOIs
Publication statusPublished - 2006 Sep
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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