TY - JOUR
T1 - Stimulated glucose uptake in the ischemic border zone
T2 - Its dependence on glucose uptake in the normally perfused area
AU - Yamane, Yuriko
AU - Ishide, Nobumasa
AU - Kagaya, Yutaka
AU - Takeyama, Daiya
AU - Shiba, Nobuyuki
AU - Chida, Masanobu
AU - Nozaki, Tetsuji
AU - Takahashi, Toshihiro
AU - Ido, Tatsuo
AU - Shirato, Kunio
PY - 1997/10
Y1 - 1997/10
N2 - During acute regional myocardial ischemia, a 'border zone' exists where the spatial distributions of blood flow and substrate uptake show gradual changes. We investigated the relationship between blood flow and glucose uptake in the border zone during acute regional ischemia. Methods: Newly developed quantitative autoradiography using imaging plates and two long- lived radioisotopes was applied to rat hearts subjected to 30 min of left coronary artery occlusion. Blood flow, glucose uptake and fatty acid uptake was assessed with 4-[N-methyl-14C]iodoantipyrine, 2-deoxy-D-[1-3H]glucose (3H-DG) and β-methyl[1-14C]heptadecanoic acid (14C-BMHDA), respectively. Results: In rats showing 3H-DG uptake in the normally perfused area (Norm) of 254 ± 96 Bq/mg (high-DG) and 56 ± 20 Bq/mg (low-DG) (n = 4 for each), 3H-DG uptake in the border zone was 148 ± 52 Bq/mg and 58 ± 15 Bq/mg (p<0.05 high- versus low-DG), respectively. The relationship between blood flow and 3H-DG uptake in the border zone was altered by the different 3H-DG uptake levels in Norm. In high-DG, 3H-DG uptake in the border zone was reduced significantly according to the decrease in the percentage of blood flow. However, in low-DG, no significant differences in 3H-DG uptake were found among the regions in the border zone with different levels of the percentage of blood flow, except in the region with 10%-19% of the percentage of blood flow. In the border zone, the percentage of 3H-DG uptake per unit blood flow normalized to that in Norm increased according to the decrease in the percentage of blood flow, and this increase was steeper in low-DG than in high-DG (p<0.0005). The percentage of 14C-BMHDA uptake was lower than the percentage of 3H-DG uptake (27 ± 3% versus 78 ± 18% of that in Norm, p<0.0005) in the peripheral ischemic area. Conclusion: The relationship between blood flow and glucose uptake in the ischemic border zone was altered by the different glucose uptake levels in Norm. Glucose uptake in the border zone was higher in rats with higher glucose uptake levels in Norm, suggesting that glucose uptake in the border zone stimulated by ischemia can be accelerated still more by humoral factors.
AB - During acute regional myocardial ischemia, a 'border zone' exists where the spatial distributions of blood flow and substrate uptake show gradual changes. We investigated the relationship between blood flow and glucose uptake in the border zone during acute regional ischemia. Methods: Newly developed quantitative autoradiography using imaging plates and two long- lived radioisotopes was applied to rat hearts subjected to 30 min of left coronary artery occlusion. Blood flow, glucose uptake and fatty acid uptake was assessed with 4-[N-methyl-14C]iodoantipyrine, 2-deoxy-D-[1-3H]glucose (3H-DG) and β-methyl[1-14C]heptadecanoic acid (14C-BMHDA), respectively. Results: In rats showing 3H-DG uptake in the normally perfused area (Norm) of 254 ± 96 Bq/mg (high-DG) and 56 ± 20 Bq/mg (low-DG) (n = 4 for each), 3H-DG uptake in the border zone was 148 ± 52 Bq/mg and 58 ± 15 Bq/mg (p<0.05 high- versus low-DG), respectively. The relationship between blood flow and 3H-DG uptake in the border zone was altered by the different 3H-DG uptake levels in Norm. In high-DG, 3H-DG uptake in the border zone was reduced significantly according to the decrease in the percentage of blood flow. However, in low-DG, no significant differences in 3H-DG uptake were found among the regions in the border zone with different levels of the percentage of blood flow, except in the region with 10%-19% of the percentage of blood flow. In the border zone, the percentage of 3H-DG uptake per unit blood flow normalized to that in Norm increased according to the decrease in the percentage of blood flow, and this increase was steeper in low-DG than in high-DG (p<0.0005). The percentage of 14C-BMHDA uptake was lower than the percentage of 3H-DG uptake (27 ± 3% versus 78 ± 18% of that in Norm, p<0.0005) in the peripheral ischemic area. Conclusion: The relationship between blood flow and glucose uptake in the ischemic border zone was altered by the different glucose uptake levels in Norm. Glucose uptake in the border zone was higher in rats with higher glucose uptake levels in Norm, suggesting that glucose uptake in the border zone stimulated by ischemia can be accelerated still more by humoral factors.
KW - Double-tracer autoradiography
KW - Imaging plate
KW - Myocardial fatty acid metabolism
KW - Myocardial glucose metabolism
KW - Regional myocardial ischemia
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M3 - Article
C2 - 9379185
AN - SCOPUS:9844220288
VL - 38
SP - 1515
EP - 1521
JO - Journal of Nuclear Medicine
JF - Journal of Nuclear Medicine
SN - 0161-5505
IS - 10
ER -