The protein band patterns of stigma extracts were differentiated in seven different S-homozygotes of self-incompatible Brassica campestris L. by polyacrylamide gel isoelectric focusing. The protein bands which differentiated S-alleles were heritable and appeared in stigmas but neither in styles nor in ovaries. The bands were thought to be glycoproteins in nature since they were PAS positive and precipitated with Con A. Such glycoproteins are postulated to be S-specific proteins. One or two glycoprotein bands were found in monogenomic self-compatible species (B. campestris var. yellow sarson and B. tournefortii). They were common in every strain within a species so far but different between two species. Digenomic self-compatible B. napus had several thin glycoprotein bands.
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