Stepwise enhancement of catalytic performance of haloalkane dehalogenase LinB towards β-hexachlorocyclohexane

Ryota Moriuchi, Hiroki Tanaka, Yuki Nikawadori, Mayuko Ishitsuka, Michihiro Ito, Yoshiyuki Ohtsubo, Masataka Tsuda, Jiri Damborsky, Zbynek Prokop, Yuji Nagata

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Two haloalkane dehalogenases, LinBUT and LinBMI, each with 296 amino acid residues, exhibit only seven amino acid residue differences between them, but LinBMI’s catalytic performance towards β-hexachlorocyclohexane (β-HCH) is considerably higher than LinBUT’s. To elucidate the molecular basis governing this difference, intermediate mutants between LinBUT and LinBMI were constructed and kinetically characterized. The activities of LinBUT-based mutants gradually increased by cumulative mutations into LinBUT, and the effects of the individual amino acid substitutions depended on combination with other mutations. These results indicated that LinBUT’s β-HCH degradation activity can be enhanced in a stepwise manner by the accumulation of point mutations.

    Original languageEnglish
    Article number72
    Pages (from-to)1-10
    Number of pages10
    JournalAMB Express
    Volume4
    Issue number1
    DOIs
    Publication statusPublished - 2014 Dec 1

    Keywords

    • Biodegradation
    • Haloalkane dehalogenase
    • Protein evolution
    • Xenobiotics
    • β-Hexachlorocyclohexane

    ASJC Scopus subject areas

    • Biophysics
    • Applied Microbiology and Biotechnology

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