STED microscopy – super-resolution bio-imaging utilizing a stimulated emission depletion

Kohei Otomo, Terumasa Hibi, Yuichi Kozawa, Tomomi Nemoto

Research output: Contribution to journalReview article

13 Citations (Scopus)

Abstract

One of the most popular super-resolution microscopies that breaks the diffraction barrier is stimulated emission depletion (STED) microscopy. As the optical set-up of STED microscopy is based on a laser scanning microscopy (LSM) system, it potentially has several merits of LSM like confocal or two-photon excitation LSM. In this article, we first describe the principles of STED microscopy and then describe the features of our newly developed two-photon excitation STED microscopy. On the basis of our recent results and those of other researchers, we conclude by discussing future research and new technologies in this field.

Original languageEnglish
Pages (from-to)227-236
Number of pages10
JournalMicroscopy
Volume64
Issue number4
DOIs
Publication statusPublished - 2015 Aug 1

Keywords

  • Liquid crystal devices
  • Optical vortex
  • Two-photon microscopy

ASJC Scopus subject areas

  • Structural Biology
  • Instrumentation
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'STED microscopy – super-resolution bio-imaging utilizing a stimulated emission depletion'. Together they form a unique fingerprint.

  • Cite this