Staphylococcus aureus giant protein Ebh is involved in tolerance to transient hyperosmotic pressure

Makoto Kuroda, Yoshikazu Tanaka, Ryo Aoki, Deng Shu, Kouhei Tsumoto, Toshiko Ohta

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Staphylococcus aureus is well known to colonize on human skin where the physiological condition is characterized by hypervariable water activity, i.e., repeated dehydration or rehydration. To determine the facilitating factors for the colonization under hypervariable water activity, we studied the giant protein Ebh (extracellular matrix (ECM)-binding protein homologue). The ebh mutant RAM8 showed invaginated vacuoles along the septum, similar to that found in partial plasmolysis, and the cells burst under osmotic upshift. RAM8 was also relatively susceptible to abrupt hyperosmotic upshift, teicoplanin, and Triton X-100. By using the green fluorescent protein (GFP) as a reporter, Ebh was localized over the entire cell surface. This suggests that Ebh might contribute to structural homeostasis by forming a bridge between the cell-wall and cytoplasmic membrane to avoid plasmolysis under hyperosmotic condition.

Original languageEnglish
Pages (from-to)237-241
Number of pages5
JournalBiochemical and biophysical research communications
Volume374
Issue number2
DOIs
Publication statusPublished - 2008 Sep 19
Externally publishedYes

Keywords

  • Cell-wall surface protein
  • Halophilic bacterium
  • Osmotic pressure
  • Salt tolerance
  • Staphylococcus aureus
  • Water activity

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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