TY - JOUR
T1 - Staphylococcus aureus giant protein Ebh is involved in tolerance to transient hyperosmotic pressure
AU - Kuroda, Makoto
AU - Tanaka, Yoshikazu
AU - Aoki, Ryo
AU - Shu, Deng
AU - Tsumoto, Kouhei
AU - Ohta, Toshiko
N1 - Funding Information:
This work was supported by a grant-in-aid for Scientific Research (C) from the Japan Society for the Promotion of Science (Grant No. 19590439).
PY - 2008/9/19
Y1 - 2008/9/19
N2 - Staphylococcus aureus is well known to colonize on human skin where the physiological condition is characterized by hypervariable water activity, i.e., repeated dehydration or rehydration. To determine the facilitating factors for the colonization under hypervariable water activity, we studied the giant protein Ebh (extracellular matrix (ECM)-binding protein homologue). The ebh mutant RAM8 showed invaginated vacuoles along the septum, similar to that found in partial plasmolysis, and the cells burst under osmotic upshift. RAM8 was also relatively susceptible to abrupt hyperosmotic upshift, teicoplanin, and Triton X-100. By using the green fluorescent protein (GFP) as a reporter, Ebh was localized over the entire cell surface. This suggests that Ebh might contribute to structural homeostasis by forming a bridge between the cell-wall and cytoplasmic membrane to avoid plasmolysis under hyperosmotic condition.
AB - Staphylococcus aureus is well known to colonize on human skin where the physiological condition is characterized by hypervariable water activity, i.e., repeated dehydration or rehydration. To determine the facilitating factors for the colonization under hypervariable water activity, we studied the giant protein Ebh (extracellular matrix (ECM)-binding protein homologue). The ebh mutant RAM8 showed invaginated vacuoles along the septum, similar to that found in partial plasmolysis, and the cells burst under osmotic upshift. RAM8 was also relatively susceptible to abrupt hyperosmotic upshift, teicoplanin, and Triton X-100. By using the green fluorescent protein (GFP) as a reporter, Ebh was localized over the entire cell surface. This suggests that Ebh might contribute to structural homeostasis by forming a bridge between the cell-wall and cytoplasmic membrane to avoid plasmolysis under hyperosmotic condition.
KW - Cell-wall surface protein
KW - Halophilic bacterium
KW - Osmotic pressure
KW - Salt tolerance
KW - Staphylococcus aureus
KW - Water activity
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U2 - 10.1016/j.bbrc.2008.07.037
DO - 10.1016/j.bbrc.2008.07.037
M3 - Article
C2 - 18639517
AN - SCOPUS:48349124615
VL - 374
SP - 237
EP - 241
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -