STAM, signal transducing adaptor molecule, is associated with janus kinases and involved in signaling for cell growth and c-myc induction

Toshikazu Takeshita, Tomikazu Arita, Masaya Higuchi, Hironobu Asao, Kazuhiro Endo, Hiroshi Kuroda, Nobuyuki Tanaka, Kazuko Murata, Naoto Ishii, Kazuo Sugamura

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123 Citations (Scopus)

Abstract

We previously identified a putative signal transducing adaptor molecule, named STAM, that contains an Src homology 3 (SH3) domain and immunoreceptor tyrosine-based activation motif (ITAM). In this report, we demonstrate the functional significance of STAM in cytokine-mediated signal transduction. STAM is associated with Jak3 and Jak2 tyrosine kinases via its ITAM region and phosphorylated by Jak3 and Jak2 upon stimulation with IL-2 and GM-CSF, respectively. An SH3 deletion mutant of STAM confers a dominant negative effect on DNA synthesis mediated by IL-2 and GM-CSF. Furthermore, the wild-type STAM, but not STAM mutants deleted of SH3 and ITAM, significantly enhances c-myc induction mediated by IL-2 and GM-CSF. These results strongly implicate STAM in the signaling pathways for cell growth and c-myc induction immediately downstream of the Jaks associated with the cytokine receptors.

Original languageEnglish
Pages (from-to)449-457
Number of pages9
JournalImmunity
Volume6
Issue number4
DOIs
Publication statusPublished - 1997 Apr 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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    Takeshita, T., Arita, T., Higuchi, M., Asao, H., Endo, K., Kuroda, H., Tanaka, N., Murata, K., Ishii, N., & Sugamura, K. (1997). STAM, signal transducing adaptor molecule, is associated with janus kinases and involved in signaling for cell growth and c-myc induction. Immunity, 6(4), 449-457. https://doi.org/10.1016/S1074-7613(00)80288-5