TY - JOUR
T1 - Staining patterns for actin and villin distinguish M cells in bovine follicle-associated epithelium
AU - Kanaya, Takashi
AU - Aso, Hisashi
AU - Miyazawa, Kohtaro
AU - Kido, Taketomo
AU - Minashima, Takeshi
AU - Watanabe, Kouichi
AU - Ohwada, Shyuichi
AU - Kitazawa, Haruki
AU - Rose, Michael T.
AU - Yamaguchi, Takahiro
N1 - Funding Information:
This study was supported by a Grant-in-Aid for Scientific Research (18658104) from the Ministry of Education, Culture, Sports, Science and Technology, and by two grant (BSE Control Project and Secure and Healthy Livestock Farming Project) from the Ministry of Agriculture, Forestry and Fisheries.
PY - 2007/4
Y1 - 2007/4
N2 - M cells play a central role in the initiation of mucosal immune responses. However, a primary source of difficulty for investigations of this is the lack of an available specific marker for bovine M cells. As M cells possess irregular and short microvilli, we investigated the distribution and localization of the microvillar proteins actin and villin by immunohistochemistry of the gut of calves. In ileum of the calf, actin and villin were clearly and continuously immunostained in the brush border of the villous epithelia, however, discontinuous immunostaining with patches of no staining were observed in follicle-associated epithelium (FAE). Electron microscopy revealed that M cells had irregular microvilli and lacked the typical brush border, and it was inferred that these patches of no staining might be the intercellular crevices of M cells. As the microvilli of M cells were very sparse, there were several areas of weak immunostaining in calf jejunal FAE. These results suggest that M cells in calf FAE are detectable by the absence of staining for actin and villin.
AB - M cells play a central role in the initiation of mucosal immune responses. However, a primary source of difficulty for investigations of this is the lack of an available specific marker for bovine M cells. As M cells possess irregular and short microvilli, we investigated the distribution and localization of the microvillar proteins actin and villin by immunohistochemistry of the gut of calves. In ileum of the calf, actin and villin were clearly and continuously immunostained in the brush border of the villous epithelia, however, discontinuous immunostaining with patches of no staining were observed in follicle-associated epithelium (FAE). Electron microscopy revealed that M cells had irregular microvilli and lacked the typical brush border, and it was inferred that these patches of no staining might be the intercellular crevices of M cells. As the microvilli of M cells were very sparse, there were several areas of weak immunostaining in calf jejunal FAE. These results suggest that M cells in calf FAE are detectable by the absence of staining for actin and villin.
KW - Actin
KW - Bovine
KW - Follicle-associated epithelium (FAE)
KW - M cell
KW - Microvilli
KW - Villin
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U2 - 10.1016/j.rvsc.2006.05.009
DO - 10.1016/j.rvsc.2006.05.009
M3 - Article
C2 - 16949627
AN - SCOPUS:33846804531
VL - 82
SP - 141
EP - 149
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
IS - 2
ER -