Staining patterns for actin and villin distinguish M cells in bovine follicle-associated epithelium

Takashi Kanaya, Hisashi Aso, Kohtaro Miyazawa, Taketomo Kido, Takeshi Minashima, Kouichi Watanabe, Shyuichi Ohwada, Haruki Kitazawa, Michael T. Rose, Takahiro Yamaguchi

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14 Citations (Scopus)

Abstract

M cells play a central role in the initiation of mucosal immune responses. However, a primary source of difficulty for investigations of this is the lack of an available specific marker for bovine M cells. As M cells possess irregular and short microvilli, we investigated the distribution and localization of the microvillar proteins actin and villin by immunohistochemistry of the gut of calves. In ileum of the calf, actin and villin were clearly and continuously immunostained in the brush border of the villous epithelia, however, discontinuous immunostaining with patches of no staining were observed in follicle-associated epithelium (FAE). Electron microscopy revealed that M cells had irregular microvilli and lacked the typical brush border, and it was inferred that these patches of no staining might be the intercellular crevices of M cells. As the microvilli of M cells were very sparse, there were several areas of weak immunostaining in calf jejunal FAE. These results suggest that M cells in calf FAE are detectable by the absence of staining for actin and villin.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalResearch in Veterinary Science
Volume82
Issue number2
DOIs
Publication statusPublished - 2007 Apr 1

Keywords

  • Actin
  • Bovine
  • Follicle-associated epithelium (FAE)
  • M cell
  • Microvilli
  • Villin

ASJC Scopus subject areas

  • veterinary(all)

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    Kanaya, T., Aso, H., Miyazawa, K., Kido, T., Minashima, T., Watanabe, K., Ohwada, S., Kitazawa, H., Rose, M. T., & Yamaguchi, T. (2007). Staining patterns for actin and villin distinguish M cells in bovine follicle-associated epithelium. Research in Veterinary Science, 82(2), 141-149. https://doi.org/10.1016/j.rvsc.2006.05.009