Stable expression of the motor protein prestin in Chinese hamster ovary cells

Koji Iida, Kazuaki Konno, Takeshi Oshima, Kouhei Tsumoto, Katsuhisa Ikeda, Izumi Kumagai, Toshimitsu Kobayashi, Hiroshi Wada

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Mammalian hearing sensitivity relies on a mechanical amplification mechanism involving the outer hair cells (OHCs), which rapidly alter their longitudinal length in response to changes in their membrane potential. The molecular basis of this mechanism is thought to be a motor protein embedded in the lateral membrane of the OHCs. Recently, this motor protein was identified and termed prestin. Since then, prestin has been researched intensively to elucidate the behavior of the OHCs. However, little progress in the study of prestin at the molecular level has been made because no method of obtaining an adequate amount of prestin has been established. In this study, therefore, an attempt was made to construct a stable expression system of prestin using Chinese hamster ovary (CHO) cells. The expression of prestin in the transfected CHO cells and the activity of prestin on CHO cells were confirmed by immunofluorescence and whole-cell patch-clamp measurements, respectively.

Original languageEnglish
Pages (from-to)1266-1274
Number of pages9
JournalJSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
Volume46
Issue number4
DOIs
Publication statusPublished - 2003 Dec

Keywords

  • Acoustic
  • Biomechanics
  • Chinese hamster ovary cell
  • Cloning
  • Measurement
  • Outer hair cell
  • Patch clamp technique
  • Prestin

ASJC Scopus subject areas

  • Mechanical Engineering
  • Industrial and Manufacturing Engineering

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