SS-A/Ro52, an autoantigen involved in CD28-mediated IL-2 production

Tomonori Ishii, Kei Ohnuma, Akikazu Murakami, Naruhiko Takasawa, Tadanori Yamochi, Satoshi Iwata, Masahiko Uchiyama, Nam H. Dang, Hirotoshi Tanaka, Chikao Morimoto

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

An autoantibody against SS-A/Ro52 (Ro52) is most frequently found in the sera of patients with Sjögren's syndrome, systemic lupus erythematosus, and congenital heart block from anti-Ro52 Ab-positive mother. However, the physiological function of the autoantigen SS-A/Ro52 has not yet been elucidated. In this study, we describe the role of Ro52 protein in T cell activation. Overexpression of SS-A/Ro52 in Jurkat T cell resulted in enhanced IL-2 production following CD28 stimulation. Furthermore, transfection of anti-Ro52-specific small RNA duplexes partially blocked the expression of native and overexpressed Ro52 in Jurkat T cell, resulting in decreased IL-2 production via CD28 pathway in these cells. Finally, intracellular localization of Ro52 dramatically changed following CD28 stimulation. Our data reveal a novel function of Ro52 in CD28-mediated pathway, which eventually contributes to cytokine production and expression of the T cell biological programs.

Original languageEnglish
Pages (from-to)3653-3661
Number of pages9
JournalJournal of Immunology
Volume170
Issue number7
DOIs
Publication statusPublished - 2003 Apr 1
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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