Abstract
Identification of small-molecule ligands for a protein of interest can facilitate the analysis of the protein's functions in biological systems. Small-molecule microarrays have allowed for rapid detection of such ligand-protein interactions in a high-throughput manner, although a label on a protein is needed to observe these interactions. By combining SPR imaging technology with our recently developed photo-cross-linked small-molecule array platform, we developed a novel platform that allows in situ observation of interactions between photo-cross-linked small molecules on gold surfaces and nonlabeled proteins in solution. Interactions of estrogenic and androgenic substances with estrogen receptor α were observed using this platform.
Original language | English |
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Pages (from-to) | 2226-2230 |
Number of pages | 5 |
Journal | Analytical Chemistry |
Volume | 78 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2006 Apr 1 |
ASJC Scopus subject areas
- Analytical Chemistry