SPR imaging of photo-cross-linked small-molecule arrays on gold

Naoki Kanoh; Motoki Kyo; Kazuki Inamori; Ami Ando; Aya Asami; Aiko Nakao; Hiroyuki Osada

doi:10.1021/ac051777j

SPR imaging of photo-cross-linked small-molecule arrays on gold

Naoki Kanoh, Motoki Kyo, Kazuki Inamori, Ami Ando, Aya Asami, Aiko Nakao, Hiroyuki Osada

PHA - Molecular Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

101 Citations (Scopus)

Abstract

Identification of small-molecule ligands for a protein of interest can facilitate the analysis of the protein's functions in biological systems. Small-molecule microarrays have allowed for rapid detection of such ligand-protein interactions in a high-throughput manner, although a label on a protein is needed to observe these interactions. By combining SPR imaging technology with our recently developed photo-cross-linked small-molecule array platform, we developed a novel platform that allows in situ observation of interactions between photo-cross-linked small molecules on gold surfaces and nonlabeled proteins in solution. Interactions of estrogenic and androgenic substances with estrogen receptor α were observed using this platform.

Original language	English
Pages (from-to)	2226-2230
Number of pages	5
Journal	Analytical Chemistry
Volume	78
Issue number	7
DOIs	https://doi.org/10.1021/ac051777j
Publication status	Published - 2006 Apr 1

ASJC Scopus subject areas

Analytical Chemistry

Access to Document

10.1021/ac051777j

Cite this

@article{fc8e9521a3cd41438ed9743d812c33f1,

title = "SPR imaging of photo-cross-linked small-molecule arrays on gold",

abstract = "Identification of small-molecule ligands for a protein of interest can facilitate the analysis of the protein's functions in biological systems. Small-molecule microarrays have allowed for rapid detection of such ligand-protein interactions in a high-throughput manner, although a label on a protein is needed to observe these interactions. By combining SPR imaging technology with our recently developed photo-cross-linked small-molecule array platform, we developed a novel platform that allows in situ observation of interactions between photo-cross-linked small molecules on gold surfaces and nonlabeled proteins in solution. Interactions of estrogenic and androgenic substances with estrogen receptor α were observed using this platform.",

author = "Naoki Kanoh and Motoki Kyo and Kazuki Inamori and Ami Ando and Aya Asami and Aiko Nakao and Hiroyuki Osada",

year = "2006",

month = apr,

day = "1",

doi = "10.1021/ac051777j",

language = "English",

volume = "78",

pages = "2226--2230",

journal = "Industrial And Engineering Chemistry Analytical Edition",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "7",

}

TY - JOUR

T1 - SPR imaging of photo-cross-linked small-molecule arrays on gold

AU - Kanoh, Naoki

AU - Kyo, Motoki

AU - Inamori, Kazuki

AU - Ando, Ami

AU - Asami, Aya

AU - Nakao, Aiko

AU - Osada, Hiroyuki

PY - 2006/4/1

Y1 - 2006/4/1

N2 - Identification of small-molecule ligands for a protein of interest can facilitate the analysis of the protein's functions in biological systems. Small-molecule microarrays have allowed for rapid detection of such ligand-protein interactions in a high-throughput manner, although a label on a protein is needed to observe these interactions. By combining SPR imaging technology with our recently developed photo-cross-linked small-molecule array platform, we developed a novel platform that allows in situ observation of interactions between photo-cross-linked small molecules on gold surfaces and nonlabeled proteins in solution. Interactions of estrogenic and androgenic substances with estrogen receptor α were observed using this platform.

AB - Identification of small-molecule ligands for a protein of interest can facilitate the analysis of the protein's functions in biological systems. Small-molecule microarrays have allowed for rapid detection of such ligand-protein interactions in a high-throughput manner, although a label on a protein is needed to observe these interactions. By combining SPR imaging technology with our recently developed photo-cross-linked small-molecule array platform, we developed a novel platform that allows in situ observation of interactions between photo-cross-linked small molecules on gold surfaces and nonlabeled proteins in solution. Interactions of estrogenic and androgenic substances with estrogen receptor α were observed using this platform.

UR - http://www.scopus.com/inward/record.url?scp=33645653692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645653692&partnerID=8YFLogxK

U2 - 10.1021/ac051777j

DO - 10.1021/ac051777j

M3 - Article

C2 - 16579601

AN - SCOPUS:33645653692

VL - 78

SP - 2226

EP - 2230

JO - Industrial And Engineering Chemistry Analytical Edition

JF - Industrial And Engineering Chemistry Analytical Edition

SN - 0003-2700

IS - 7

ER -

SPR imaging of photo-cross-linked small-molecule arrays on gold

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this