SPR imaging of photo-cross-linked small-molecule arrays on gold

Naoki Kanoh, Motoki Kyo, Kazuki Inamori, Ami Ando, Aya Asami, Aiko Nakao, Hiroyuki Osada

Research output: Contribution to journalArticlepeer-review

97 Citations (Scopus)

Abstract

Identification of small-molecule ligands for a protein of interest can facilitate the analysis of the protein's functions in biological systems. Small-molecule microarrays have allowed for rapid detection of such ligand-protein interactions in a high-throughput manner, although a label on a protein is needed to observe these interactions. By combining SPR imaging technology with our recently developed photo-cross-linked small-molecule array platform, we developed a novel platform that allows in situ observation of interactions between photo-cross-linked small molecules on gold surfaces and nonlabeled proteins in solution. Interactions of estrogenic and androgenic substances with estrogen receptor α were observed using this platform.

Original languageEnglish
Pages (from-to)2226-2230
Number of pages5
JournalAnalytical Chemistry
Volume78
Issue number7
DOIs
Publication statusPublished - 2006 Apr 1

ASJC Scopus subject areas

  • Analytical Chemistry

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