TY - JOUR
T1 - Sporotomaculum syntrophicum sp. nov., a novel anaerobic, syntrophic benzoate-degrading bacterium isolated from methanogenic sludge treating wastewater from terephthalate manufacturing
AU - Qiu, Yan Ling
AU - Sekiguchi, Yuji
AU - Imachi, Hiroyuki
AU - Kamagata, Yoichi
AU - Tseng, I. Cheng
AU - Cheng, Sheng Shung
AU - Ohashi, Akiyoshi
AU - Harada, Hideki
N1 - Funding Information:
Acknowledgements We thank the following researchers in the National Institute of Advanced Industrial Science and Technology (AIST): Xian-Ying Meng for transmission electron microscopy and Aiko Sukegawa for the determinations of quinones and the DNA base composition. This study was financially supported by research grant no.11794002 as part of the grant-in-aid for University and Society Collaboration from the Ministry of Education, Culture, Sports, Science and Technology, Japan, as well as by an international research grant from the Interchange Association (IA), Japan.
PY - 2003/4/1
Y1 - 2003/4/1
N2 - An anaerobic, mesophilic, syntrophic benzoate-degrading bacterium, designated strain FBT, was isolated from methanogenic sludge which had been used to treat wastewater from the manufacture of terephthalic acid. Cells were non-motile gram-positive rods that formed spores. The optimum temperature for growth was 35-40 °C, and the optimum pH was 7.0-7.2. A co-culture with the hydrogenotrophic methanogen Methanospirillum hungatei converted benzoate to acetate, carbon dioxide, and methane. Butyrate transiently accumulated at a high concentration of 2.5 mM during degradation. Besidesbenzoate, no other compound tested supported growth of the co-culture. Crotonate supported growth of strain FBT in pure culture. Furthermore, the strain degraded benzoate in pure culture with crotonate as co-substrate to produce acetate and butyrate. The strain was not able to utilize sulfate, sulfite, thiosulfate, nitrate, fumarate, or Fe(III) as electron acceptor. The G+C content of the DNA was 46.8 mol%. Strain FBT contained MK-7 as the major quinone and C16:1 as the major fatty acid. 16S rDNA sequence analysis revealed that the strain was a member of the genus Sporotomaculum, even though it exhibited significant differences, such as the capacity for syntrophic growth, to the known member of the genus. Hence, we propose the name Sporotomaculum syntrophicum sp. nov. for strain FBT. The type strain is strain FBT (DSM 14795, JCM 11475).
AB - An anaerobic, mesophilic, syntrophic benzoate-degrading bacterium, designated strain FBT, was isolated from methanogenic sludge which had been used to treat wastewater from the manufacture of terephthalic acid. Cells were non-motile gram-positive rods that formed spores. The optimum temperature for growth was 35-40 °C, and the optimum pH was 7.0-7.2. A co-culture with the hydrogenotrophic methanogen Methanospirillum hungatei converted benzoate to acetate, carbon dioxide, and methane. Butyrate transiently accumulated at a high concentration of 2.5 mM during degradation. Besidesbenzoate, no other compound tested supported growth of the co-culture. Crotonate supported growth of strain FBT in pure culture. Furthermore, the strain degraded benzoate in pure culture with crotonate as co-substrate to produce acetate and butyrate. The strain was not able to utilize sulfate, sulfite, thiosulfate, nitrate, fumarate, or Fe(III) as electron acceptor. The G+C content of the DNA was 46.8 mol%. Strain FBT contained MK-7 as the major quinone and C16:1 as the major fatty acid. 16S rDNA sequence analysis revealed that the strain was a member of the genus Sporotomaculum, even though it exhibited significant differences, such as the capacity for syntrophic growth, to the known member of the genus. Hence, we propose the name Sporotomaculum syntrophicum sp. nov. for strain FBT. The type strain is strain FBT (DSM 14795, JCM 11475).
KW - Anaerobic benzoate oxidation
KW - Sporotomaculum syntrophicum sp. nov
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U2 - 10.1007/s00203-003-0521-z
DO - 10.1007/s00203-003-0521-z
M3 - Article
C2 - 12605290
AN - SCOPUS:0242500845
VL - 179
SP - 242
EP - 249
JO - Archives of Microbiology
JF - Archives of Microbiology
SN - 0302-8933
IS - 4
ER -