Sporotomaculum syntrophicum sp. nov., a novel anaerobic, syntrophic benzoate-degrading bacterium isolated from methanogenic sludge treating wastewater from terephthalate manufacturing

Yan Ling Qiu, Yuji Sekiguchi, Hiroyuki Imachi, Yoichi Kamagata, I. Cheng Tseng, Sheng Shung Cheng, Akiyoshi Ohashi, Hideki Harada

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

An anaerobic, mesophilic, syntrophic benzoate-degrading bacterium, designated strain FBT, was isolated from methanogenic sludge which had been used to treat wastewater from the manufacture of terephthalic acid. Cells were non-motile gram-positive rods that formed spores. The optimum temperature for growth was 35-40 °C, and the optimum pH was 7.0-7.2. A co-culture with the hydrogenotrophic methanogen Methanospirillum hungatei converted benzoate to acetate, carbon dioxide, and methane. Butyrate transiently accumulated at a high concentration of 2.5 mM during degradation. Besidesbenzoate, no other compound tested supported growth of the co-culture. Crotonate supported growth of strain FBT in pure culture. Furthermore, the strain degraded benzoate in pure culture with crotonate as co-substrate to produce acetate and butyrate. The strain was not able to utilize sulfate, sulfite, thiosulfate, nitrate, fumarate, or Fe(III) as electron acceptor. The G+C content of the DNA was 46.8 mol%. Strain FBT contained MK-7 as the major quinone and C16:1 as the major fatty acid. 16S rDNA sequence analysis revealed that the strain was a member of the genus Sporotomaculum, even though it exhibited significant differences, such as the capacity for syntrophic growth, to the known member of the genus. Hence, we propose the name Sporotomaculum syntrophicum sp. nov. for strain FBT. The type strain is strain FBT (DSM 14795, JCM 11475).

Original languageEnglish
Pages (from-to)242-249
Number of pages8
JournalArchives of Microbiology
Volume179
Issue number4
DOIs
Publication statusPublished - 2003 Apr 1

Keywords

  • Anaerobic benzoate oxidation
  • Sporotomaculum syntrophicum sp. nov

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

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