Spontaneous single-channel activity of neuronal TRP5 channel recombinantly expressed in HEK293 cells

Hisanobu Yamada, Minoru Wakamori, Yuji Hara, Yasuo Takahashi, Kazuhiko Konishi, Keiji Imoto, Yasuo Mori

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Mammalian homologues of the Drosophila transient receptor potential (trp) protein (TRP) form Ca2+ permeable cation channels activated in response to stimulation of G-protein-coupled receptors. Establishing biophysical characteristics of basal TRP activity is of great importance in understanding modulatory processes, which underlie enhancement of TRP activity via receptor stimulation. We have examined spontaneous activity of the TRP5 channel recombinantly expressed in human embryonic kidney cells, using the conventional whole-cell mode of the patch-clamp technique in a low- Ca2+ external solution. The unitary Na+ conductance of the TRP5 channel was linear, being 47.6 pS. By contrast, the open probability of the TRP5 channel showed a voltage-dependent decrease below -50 mV. These biophysical properties are important hallmarks in distinguishing the TRP5 channel in native neuronal preparations, whose spontaneous activity may contribute to control of resting membrane potentials and generation of action potentials. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)111-114
Number of pages4
JournalNeuroscience Letters
Volume285
Issue number2
DOIs
Publication statusPublished - 2000 May 12
Externally publishedYes

Keywords

  • Cation channel
  • HEK293 cell
  • Patch clamp
  • Receptor-activated channel
  • Single-channel recording
  • Transient receptor potential protein

ASJC Scopus subject areas

  • Neuroscience(all)

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