Spontaneous calcium changes in striatal cells

Makoto Osanai, Yuichi Yaguchi, Naohiro Yamada, Fumito Oboshi, Tetsuya Yagi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The striatum plays an important role in linking cortical activity to basal ganglia outputs. We conducted the calcium (Ca2+) imaging to investigate the spontaneous activities of the striatum using acute slice preparations. The corticostriatal slices of rats were stained with Fura-PE3-AM. Long lasting spontaneous intracellular Ca2+ ([Ca2+]i) changes, which lasted up to several hundreds seconds, were observed. The amplitudes and the intervals of the changes were variable even in a single cell. Most cells exhibited irregular frequencies, but some exhibited oscillatory features. Most of these [Ca2+]i changes were not suppressed by TTX, blocker of action potentials. The number of the active cells, which exhibited the [Ca2+]i changes, was greatly reduced by the intracellular Ca 2+ store depletor, thapsigargin. Therefore, the intracellular Ca 2+ store is likely to contribute to the [Ca2+]i transients. The [Ca2+]i changes under standard ACSF and TTX showed different levels of regularity. We tested synchronization of the [Ca 2+]i changes between cell pairs under the both conditions. The number of synchronized cell pairs reduced in TTX. These results suggested that TTX insensitive and slow rate [Ca2+]i changes might exert the information processing in the striatum.

Original languageEnglish
JournalIEEJ Transactions on Electronics, Information and Systems
Volume128
Issue number7
Publication statusPublished - 2008 Jan 1
Externally publishedYes

Keywords

  • Calciumion
  • Glial cell
  • Imaging
  • Neuron
  • Spontaneous activity
  • Time series analysis

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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  • Cite this

    Osanai, M., Yaguchi, Y., Yamada, N., Oboshi, F., & Yagi, T. (2008). Spontaneous calcium changes in striatal cells. IEEJ Transactions on Electronics, Information and Systems, 128(7).