Spontaneous calcium changes in striatal cells

Makoto Osanai, Yuichi Yaguchi, Naohiro Yamada, Fumito Oboshi, Tetsuya Yagi

Research output: Contribution to journalArticlepeer-review

Abstract

The striatum plays an important role in linking cortical activity to basal ganglia outputs. We performed calcium (Ca2+) imaging to investigate the spontaneous activities of the striatum using acute slice preparations. Corticostriatal slices of rats were stained with Fura-PE3-AM. Long -lasting spontaneous intracellular Ca2+ ([Ca2+]i) changes, which lasted up to several hundred seconds, were observed. The amplitudes and the intervals of the changes were variable even in a single cell. Most cells exhibited irregular frequencies, but some exhibited oscillatory features. Most of these [Ca2+]i changes were not suppressed by TTX, a blocker of action potentials. The number of active cells, which exhibited the [Ca2+]i changes, was greatly reduced by the intracellular Ca2+ store depletor thapsigargin. Therefore, the intracellular Ca2+ store is likely to contribute to the [Ca 2+]i transients. The [Ca2+]i changes under standard ACSF and TTX showed different levels of regularity. We tested synchronization of the [Ca2+]i changes between cell pairs under both conditions. The number of synchronized cell pairs was reduced in TTX. These results suggest that TTX-insensitive and slow rate [Ca2+] i changes might be involved in information processing in the striatum.

Original languageEnglish
Pages (from-to)43-52
Number of pages10
JournalElectronics and Communications in Japan
Volume94
Issue number7
DOIs
Publication statusPublished - 2011 Jul

Keywords

  • calcium ion
  • glial cell
  • imaging
  • neuron
  • spontaneous activity
  • time series analysis

ASJC Scopus subject areas

  • Signal Processing
  • Physics and Astronomy(all)
  • Computer Networks and Communications
  • Electrical and Electronic Engineering
  • Applied Mathematics

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