Spontaneous and Luminol-Dependent Chemiluminescences from Tissue Preparations of Benzo[a]pyrene-Injected Mice

Lucia S. Yoshida, Teruo Miyazawa, Kenshiro Fujimoto, Takashi Kaneda

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Chemiluminescence (CL) from tissue preparations of mice i.p. injected with and without the chemical carcinogen benzo[α]pyrene (BP) was detected by a single photon counting apparatus. The spontaneously emitted CL (spontaneous CL) and the CL after luminol addition (luminol-CL) were measured for mice with and without previous induction of their liver mixed-function oxidases (MFO) by phénobarbital. In MFO-non-induced mice, although the spontaneous CL was not notably modified by BP injection, the kidneys presented three times greater luminol-CL after BP injection. On the other hand, MFO-induced mice had higher spontaneous CL of plasma, liver, kidneys, and lungs, as well as higher luminol-CL of liver after BP injection, when compared with the respective MFO-induced but BP-non-injected mice. The luminol-CL of liver was suppressed by scavengers of active oxygen and free-radicals such as TIRON, butylhydroxytoluene and in a less extent by superoxide dismutase. The CL detected from tissue preparations of mice after BP treatment is thought to reflect the formation of oxygen radicals and electronically excited-species during BP metabolism.

Original languageEnglish
Pages (from-to)569-578
Number of pages10
JournalJournal of nutritional science and vitaminology
Volume35
Issue number6
DOIs
Publication statusPublished - 1989 Jan 1

Keywords

  • benzo[a]pyrene
  • carcinogen metabolism
  • chemiluminescence
  • free-radical
  • luminol
  • mice
  • mixed-function oxidases

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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