TY - JOUR
T1 - Sphingomonas oligophenolica sp. nov., a halo- and organo-sensitive oligotrophic bacterium from paddy soil that degrades phenolic acids at low concentrations
AU - Ohta, Hiroyuki
AU - Hattori, Reiko
AU - Ushiba, Yuuji
AU - Mitsui, Hisayuki
AU - Ito, Masao
AU - Watanabe, Hiroshi
AU - Tonosaki, Akira
AU - Hattori, Tsutomu
PY - 2004/11
Y1 - 2004/11
N2 - The taxonomic position of a halo- and organo-sensitive, oligotrophic soil bacterium, strain S213T was investigated. Cells were Gram-negative, non-motile, strictly aerobic, yellow-pigmented rods of short to medium length on diluted nutrient broth. When 0.1-0.4% (w/v) NaCl was added to diluted media composed of peptone and meat extract, growth was inhibited with increasing NaCl concentration and the cells became long aberrant forms. When 6 mM CaCl2 was added, the cells grew quite normally and aberrant cells were no longer found at 0.1-0.5% (w/v) NaCl. Chemotaxonomically, strain S213T contains chemical markers that indicate its assignment to the Sphingomonadaceae: the presence of ubiquinone Q-10 as the predominant respiratory quinone, C18:1 and C16:0 as major fatty acids, C14:0 2-OH as the major 2-hydroxy fatty acid and sphingoglycolipids. 16S rRNA gene sequence analysis indicated that strain S213T belongs to the genus Sphingomonas, exhibiting high sequence similarity to the 16S rRNA gene sequences of Sphingomonas mali IFO 15500T (98.3%), Sphingomonas pruni IFO 15498T (98.0%), Sphingomonas asaccharolytica IFO 15499T (97.9%) and Sphingomonas echinoides DSM 1805T (97.8%). The results of DNA-DNA hybridization experiments and its phenotypic characteristics clearly distinguished the strain from its nearest neighbours and demonstrate that strain S213T represents a novel Sphingomonas species, for which the name Sphingomonas oligophenolica sp. nov. is proposed. The type strain is S213T (=JCM 12082T=CIP 107926T).
AB - The taxonomic position of a halo- and organo-sensitive, oligotrophic soil bacterium, strain S213T was investigated. Cells were Gram-negative, non-motile, strictly aerobic, yellow-pigmented rods of short to medium length on diluted nutrient broth. When 0.1-0.4% (w/v) NaCl was added to diluted media composed of peptone and meat extract, growth was inhibited with increasing NaCl concentration and the cells became long aberrant forms. When 6 mM CaCl2 was added, the cells grew quite normally and aberrant cells were no longer found at 0.1-0.5% (w/v) NaCl. Chemotaxonomically, strain S213T contains chemical markers that indicate its assignment to the Sphingomonadaceae: the presence of ubiquinone Q-10 as the predominant respiratory quinone, C18:1 and C16:0 as major fatty acids, C14:0 2-OH as the major 2-hydroxy fatty acid and sphingoglycolipids. 16S rRNA gene sequence analysis indicated that strain S213T belongs to the genus Sphingomonas, exhibiting high sequence similarity to the 16S rRNA gene sequences of Sphingomonas mali IFO 15500T (98.3%), Sphingomonas pruni IFO 15498T (98.0%), Sphingomonas asaccharolytica IFO 15499T (97.9%) and Sphingomonas echinoides DSM 1805T (97.8%). The results of DNA-DNA hybridization experiments and its phenotypic characteristics clearly distinguished the strain from its nearest neighbours and demonstrate that strain S213T represents a novel Sphingomonas species, for which the name Sphingomonas oligophenolica sp. nov. is proposed. The type strain is S213T (=JCM 12082T=CIP 107926T).
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U2 - 10.1099/ijs.0.02959-0
DO - 10.1099/ijs.0.02959-0
M3 - Article
C2 - 15545456
AN - SCOPUS:9744263955
VL - 54
SP - 2185
EP - 2190
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
SN - 1466-5026
IS - 6
ER -